# US 2010/0167391 A1 · Growth factor homolog zvegf3

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- Number: US 2010/0167391 A1
- Document ID: US20100167391A1
- Status: Published application
- Kind: A1
- Application number: 12/107,571
- Filed: 2008-04-22
- Published: 2010-07-01
- Earliest claimed: 1998-12-07
- Assignees: ZymoGenetics, Inc.
- Inventors: Zeren Gao; Charles E. Hart; Christopher S. Piddington; Paul O. Sheppard; Kimberty E. Shoemaker; Debra G. Gilbertson; James W. West
- Web page: https://patentgrep.com/patent/US20100167391A1

## CPC

- C07K 14/52: Cytokines; Lymphokines; Interferons
- A61K 38/1858: {Platelet-derived growth factor [PDGF]}
- C07K 14/475: Growth factors; Growth regulators
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- C12N 2799/022: where the vector is derived from an adenovirus
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)

## Abstract

Polypeptide growth factors, methods of making them, polynucleotides encoding them, antibodies to them, and methods of using them are disclosed. The polypeptides comprise an amino acid segment that is at least 90% identical to residues 46-163 of SEQ ID NO:2 or residues 235-345 of SEQ ID NO:2. Multimers of the polypeptides are also disclosed. The polypeptides, multimeric proteins, and polynucleotides can be used in the study and regulation of cell and tissue development, as components of cell culture media, and as diagnostic agents.

## Claims

### Claim 1

A method of activating a cell-surface PDGF alpha receptor, comprising
exposing a cell comprising a cell-surface PDGF alpha receptor to a dimeric protein having two polypeptide chains, wherein each of the polypeptide chains is from 111 to 136 amino acid residues in length and comprises a sequence of amino acid residues having at least 95% sequence identity with residues 235-345 of SEQ ID NO:2, wherein the protein is optionally glycosylated, and wherein the protein is capable of binding the PDGF alpha receptor.

### Claim 2 (depends on 1)

The method of claim 1, wherein each of the polypeptide chains is from 111 to 136 amino acid residues in length and comprises amino acid residues 235-345 of SEQ ID NO:2.

### Claim 3 (depends on 1)

The method of claim 1, wherein each of the polypeptide chains consists of a sequence of amino acid residues selected from the group consisting of:
residues 230-345 of SEQ ID NO:2;
residues 231-345 of SEQ ID NO:2;
residues 232-345 of SEQ ID NO:2;
residues 233-345 of SEQ ID NO:2;
residues 234-345 of SEQ ID NO:2;
residues 235-345 of SEQ ID NO:2;
residues 236-345 of SEQ ID NO:2;
residues 237-345 of SEQ ID NO:2;
residues 238-345 of SEQ ID NO:2;
residues 239-345 of SEQ ID NO:2; and
residues 240-345 of SEQ ID NO:2.