# US 2010/0167382 A1 · Method and device for the formation of biological cell material

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- Number: US 2010/0167382 A1
- Document ID: US20100167382A1
- Status: Published application
- Kind: A1
- Application number: 12/719,338
- Filed: 2010-03-08
- Published: 2010-07-01
- Earliest claimed: 2003-02-21
- Assignees: Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
- Inventors: Günter R. FUHR; Heiko ZIMMERMANN
- Web page: https://patentgrep.com/patent/US20100167382A1

## CPC

- B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids}
- C12M 33/02: {by impregnation, e.g. using swabs or loops (fluid transport using swabs B01L3/5029)}
- C12M 35/00: {Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion (machines for extracting juice from animal or plant tissue by electroplasmolysis A23N1/006, processes employing electric or wave energy B01J19/08; treatment of microorganisms or enzymes with electrical or wave energy C12N13/00; methods for cell fusion C12N15/02; introduction of foreign genetic material C12N15/87)}
- G01N 15/12: by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
- G01N 15/14: Optical investigation techniques, e.g. flow cytometry
- A61B 10/0233: {Pointed or sharp biopsy instruments}
- A61B 10/0283: {with vacuum aspiration, e.g. caused by retractable plunger or by connected syringe (A61B10/025, A61B10/0266 take precedence)}
- A61B 2010/0208: {Biopsy devices with actuators, e.g. with triggered spring mechanisms}
- B01L 2200/0647: Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L 2300/0838: Capillaries
- B01L 2400/0415: electrical forces, e.g. electrokinetic
- B01L 2400/043: magnetic forces
- B01L 2400/0487: fluid pressure, pneumatics
- G01N 1/286: {involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising (microtomes G01N1/06; pulverising in general B02C; mixing in general B01F)}
- G01N 2015/1486: {Counting the particles}
- G01N 2015/1493: {Particle size}
- B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- C12M: APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; {APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS}
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A61B: DIAGNOSIS; SURGERY; IDENTIFICATION (usefulness limited to only animals A61D)

## Abstract

A manipulation tool is disclosed for producing cell material having multiple biological cells, which have a predefined geometrical arrangement. The tool includes a tool body, whose surface at least partially contacts the cell material, and a setting device for adjusting the tool body by a continuous expansion, so that geometrical properties of the surface change and an interior of the tool body is enlarged. The setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 μm/h to 1 mm/h.

## Claims

### Claim 1

A manipulation tool for producing a hollow cell material comprising multiple biological cells having a predefined geometrical arrangement, said manipulation tool comprising:
a hollow tool body having at least one surface, said surface being arranged for accommodating at least one cell layer of adherently growing cells; and
a setting device arranged for an adjustment of the tool body by a continuous expansion, so that geometrical properties of the at least one surface change, and an interior of the tool body is enlarged,
wherein the setting device is adapted to expand the tool body at an advance velocity in a range from 0.1 μm/h to 1 mm/h.

### Claim 2 (depends on 1)

The manipulation tool according to claim 1, wherein the advance velocity is less than or equal to a physiological reference velocity of the cells.

### Claim 3 (depends on 1)

The manipulation tool according to claim 1, wherein the tool body comprises an elastic material.

### Claim 4 (depends on 1)

The manipulation tool according to claim 1, wherein the tool body comprises a non-elastic, expandable material.

### Claim 5 (depends on 1)

The manipulation tool according to claim 1, wherein the tool body is connected to a pressure source via a pressure line.

### Claim 6 (depends on 1)

The manipulation tool according to claim 1, wherein the tool body contains at least one displaceable shaping element.

### Claim 7 (depends on 6)

The manipulation tool according to claim 6, wherein the tool body contains multiple displaceable shaping elements, which are positioned in linear rows and columns in a matrix.

### Claim 8 (depends on 6)

The manipulation tool according to claim 6, wherein at least one cover element, which forms the at least one surface, is positioned on the at least one shaping element.

### Claim 9 (depends on 1)

The manipulation tool according to claim 1, wherein a structure or coating, which encourages adherent adhesion of biological cells, is provided on the at least one surface.

### Claim 10 (depends on 1)

The manipulation tool according to claim 1, wherein a structure or coating which blocks adherent adhesion of biological cells is provided on the surface.