# US 2010/0167350 A1 · Microorganism with ability to produce deoxy polyol dehydrogenase and use thereof

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- Number: US 2010/0167350 A1
- Document ID: US20100167350A1
- Status: Published application
- Kind: A1
- Application number: 12/515,602
- Filed: 2007-03-27
- Published: 2010-07-01
- Earliest claimed: 2006-11-20
- Assignees: Nat'l University Corporation Kagawa University; Rare Sugar Production Technical Research Labs.; Matsutani Chemical Industry Co., Ltd.
- Inventors: Ken Izumori; Kenji Morimoto; Goro Takata; Masaaki Tokuda; Yoshio Tsujisaka; Kei Takeshita; Keiji Tsusaki; Kazuhiro Okuma
- Web page: https://patentgrep.com/patent/US20100167350A1

## CPC

- C12N 1/205: Bacterial isolates
- A61K 8/60: Sugars; Derivatives thereof
- A61Q 19/00: Preparations for care of the skin
- C12N 9/0006: {acting on CH-OH groups as donors (1.1)}
- C12P 19/02: Monosaccharides (2-ketogulonic acid C12P7/60)
- A61K 2800/86: Products or compounds obtained by genetic engineering
- C12R 2001/01: Bacteria or Actinomycetales {; using bacteria or Actinomycetales}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- A61Q: SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)}
- C12R: INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS

## Abstract

Problem: To provide a microorganism with an ability to produce deoxy polyol dehydrogenase. Means for Resolution: A microorganism belonging to genus Enterobacter with an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol. The bacterial cell IK7 of the genus Enterobacter (accession No. NITE P-271). A method for producing deoxy ketose comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by the culturing of the microorganism of the invention or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol to oxidize deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose. The deoxy polyol is 1-deoxy-D-allitol, while the corresponding deoxy ketose is 1-deoxy-L-psicose. Otherwise, the deoxy polyol is L-rhamnitol, while the corresponding deoxy ketose is 1-deoxy-L-fructose.

## Claims

### Claim 1

A microorganism belonging to Enterobacter, which has an ability to produce a dehydrogenase for deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol.

### Claim 2 (depends on 1)

A microorganism according to claim 1, which belongs to Enterobacter aerogenes.

### Claim 3 (depends on 1)

A microorganism according to claim 1, which is a bacterial strain IK7 (accession No. NITE BP-271) of the genus Enterobacter.

### Claim 4 (depends on 1)

A microorganism according to claim 1, where the deoxy polyol is 1-deoxy D-allitol.

### Claim 5 (depends on 1)

A microorganism according to claim 1, where the deoxy polyol is L-rhamnitol.

### Claim 6 (depends on 1)

A method for producing deoxy ketose, comprising allowing a culture containing the deoxy polyol dehydrogenase obtained by culturing any microorganism according to claim 1 or allowing the deoxy polyol dehydrogenase to react with a solution containing deoxy polyol of the same structure at the positions C2 and C3 as that of ribitol or L-iditol, to oxidize the deoxy polyol to produce the corresponding deoxy ketose and then collecting the deoxy ketose.

### Claim 7 (depends on 6)

A method for producing deoxy ketose according to claim 6, where the deoxy polyol dehydrogenase is immobilized for reaction therewith.

### Claim 8 (depends on 6)

A method for producing deoxy ketose according to claim 6, where the deoxy polyol is 1-deoxy D-allitol and the corresponding deoxy ketose is 1-deoxy-D-psicose.

### Claim 9 (depends on 6)

A method for producing deoxy ketose according to claim 6, where the deoxy polyol is L-rhamnitol and the corresponding deoxy ketose is 1-deoxy-L-fructose.

### Claim 10 (depends on 9)

A method for producing deoxy ketose according to claim 9, where the solution containing L-rhamnitol is a reaction mixture containing L-rhamnitol as obtained by reducing L-rhamnose.

### Claim 11

1-Deoxy-L-fructose.

### Claim 12 (depends on 11)

1-Deoxy-L-fructose according to claim 11, as obtained by allowing a bacterial cell with an ability to produce deoxy polyol dehydrogenase as obtained by culturing the bacterial strain IK7 (accession No. NITE BP-271) of the genus Enterobacter to react with a solution containing L-rhamnitol to oxidize L-rhamnitol, to produce 1-deoxy-L-fructose.