Orf11 from campylobacter jejuni is a sialate-o-acetyltransferase
AssigneeNational Research Council of Canada
InventorsMichael Gilbert, Warren Wakarchuk, Scott Houliston
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Abstract
The invention relates to sialate-O-acetyltransferase (SOAT) polypeptides, nucleic acids that encode the polypeptides, and methods of using the polypeptides.
Claims27
A reaction mixture comprising a recombinant sialate-β-acetyltransferase (SOAT) polypeptide, wherein the SOAT polypeptide comprises an amino acid sequence with at least 80% identity to SEQ ID NO:10 and wherein the SOAT polypeptide transfers an acetyl moiety from a donor substrate to an acceptor substrate.
A method of making an oligosaccharide that comprises an acetylated sialic acid moiety, the method comprising the steps of
- a) contacting a sialylated oligosaccharide with a donor substrate comprising an acetyl moiety and a recombinant sialate-O-acetyltransferase (SOAT) polypeptide that comprises an amino acid sequence with at least 80% identity to SEQ ID NO:10;
- and
- b) allowing transfer of an acetyl moiety to the sialylated oligosaccharide to occur, thereby producing the oligosaccharide that comprises an acetylated sialic acid moiety.
A recombinant sialate-O-acetyltransferase (SOAT) polypeptide, wherein the SOAT polypeptide comprises an amino acid sequence with at least 99% identity to SEQ ID NO:10 and wherein the SOAT polypeptide transfers an acetyl moiety from a donor substrate to an acceptor substrate.
Dependencies are inferred from the claim text.