Novel protein expression system
AssigneeDNAVEC Corporation
InventorsJun You, Toshiaki Tabata, Makoto Inoue, Tsugumine Shu, Mamoru Hasegawa
Life of this patent
Estimating the term…
Abstract
The present inventors devised a protein expression system with coexisting MiniSeV and SeV particles, and assessed the system for the ability to transfer a gene(s) of interest into target cells, and to express the gene(s) in the target cells. It was shown that the expression system of the present invention had a high ability to transfer a gene(s) of interest into target cells, and a high ability to express the gene(s) in the target cells.
Claims22
A method of expressing a foreign gene(s) in target cells, said method comprising the steps of:
- (a) introducing into production cells a helper minus-strand RNA viral vector and a minus-strand RNA viral minigenome that carries the foreign gene(s);
- (b) collecting viral particles in which either the helper minus-strand RNA viral vector or the minus-strand RNA viral minigenome is packaged, wherein the vector and the minigenome are produced in said production cells;
- and
- (c) introducing into the target cells the viral particles collected in step (b).
A helper minus-strand RNA viral vector comprising a single-stranded minus-strand RNA that has been altered so as not to express one or more of paramyxovirus F, M, and HN proteins, and wherein the 5′-end trailer region of the single-stranded minus-strand RNA has been replaced with a leader region.
A minus-strand RNA viral minigenome that carries a foreign gene(s).
A method of producing viral particles, said method comprises the steps of:
- (a) introducing into production cells a helper minus-strand RNA viral vector and a minus-strand RNA viral minigenome that carries a foreign gene(s);
- and
- (b) collecting viral particles in which either the helper minus-strand RNA viral vector or the minus-strand RNA viral minigenome is packaged, wherein the vector and the minigenome are produced in said production cells.
Dependencies are inferred from the claim text.