# US 2010/0167309 A1 · Apparatus and method for analyte detection > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2010/0167309 A1 - Document ID: US20100167309A1 - Status: Published application - Kind: A1 - Application number: 12/723,560 - Filed: 2010-03-12 - Published: 2010-07-01 - Earliest claimed: 1997-10-06 - Assignees: Enterix, Inc. - Inventors: Howard Milne Chandler - Web page: https://patentgrep.com/patent/US20100167309A1 ## CPC - G01N 33/54388: {based on lateral flow} - G01N 33/54366: {Apparatus specially adapted for solid-phase testing} - Y10S 435/805: Test papers - Y10S 435/81: Packaged device or kit - Y10S 435/97: Test strip or test slide - Y10S 436/805: Optical property - Y10S 436/809: Multifield plates or multicontainer arrays - Y10S 436/81: Tube, bottle, or dipstick - Y10S 436/813: Cancer - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) - Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS ## Abstract Disclosed is a testing device and methods for the identification of an analyte of interest in a sample. In a preferred embodiment, the testing device includes a front panel having at least one sample application aperture; a rear panel having at least one solvent application aperture; a sample collection matrix disposed between the rear panel and the front panel, the sample collection matrix being in communication with the sample and solvent application apertures of the front and rear panels; and at least one insertable test strip containing a reagent enabling detection of the analyte of interest. ## Claims ### Claim 1 A method for identification of an analyte of interest, comprising: a. providing a testing device comprising a housing, wherein said housing comprises a) a sample application matrix comprising a desiccated sample, and b) at least one aperture in said housing to receive a testing element and hold said testing element in direct liquid-conductive communication with the sample application matrix; b. inserting at least one testing element to said at least one aperture in said housing; c. rehydrating said desiccated sample by applying sufficient solvent such that an analyte of said sample is resolubilized or resuspended, and wherein said testing element is contacted by said analyte; and d. identifying said analyte using said testing element. ### Claim 2 (depends on 1) The method of claim 1, wherein said analyte is an antigen or hapten. ### Claim 3 (depends on 2) The method of claim 2, wherein said antigen or hapten is a diagnostic indicator of a disease state in an organism. ### Claim 4 (depends on 3) The method of claim 3, wherein said disease state is selected from cancer and pathogenic infection. ### Claim 5 (depends on 3) The method of claim 3, wherein said disease state is characterized by occult gastrointestinal bleeding. ### Claim 6 (depends on 2) The method of claim 2, wherein said antigen or hapten is a contaminant in food or water. ### Claim 7 (depends on 1) The method of claim 1, wherein at least one of said testing elements is as an immunochromatographic test strip. ### Claim 8 (depends on 7) The method of claim 7, wherein said immunochromatographic test strip comprises a contact zone which, following insertion, contacts said sample application matrix and conducts a solvent front from said sample application matrix through a detection zone which contains a specific binding reagent, said detection zone being spatially distinct from said contact zone. ### Claim 9 (depends on 1) The method of claim 1, wherein said sample is selected from the group consisting of blood, urine, saliva, and fecal material. ### Claim 10 (depends on 9) The method of claim 9, wherein said fecal material is obtained by sampling toilet bowl water. ### Claim 11 A method for identification of an analyte from a plurality of samples, comprising: a. providing a testing device comprising a housing, wherein said housing comprises a) a sample application matrix, wherein said sample application matrix comprises a plurality of desiccated samples applied at discrete locations on said sample application matrix, and b) an aperture in said housing to receive a insertable testing element and hold said testing element in direct liquid-conductive communication with said sample application matrix; wherein said sample application matrix and said aperture are configured such that said plurality of samples are combined and flow onto said testing element upon rehydration of said samples; b. inserting an insertable testing element into said aperture in said housing; c. rehydrating said desiccated samples by applying sufficient solvent, wherein analytes from said samples are aggregated and flow onto said testing element; d. identifying said analyte in the aggregated sample using said testing element. ### Claim 12 (depends on 11) The method of claim 11, wherein said plurality of samples are liquid. ### Claim 13 (depends on 11) The method of claim 11, wherein said sample is selected from the group consisting of blood, urine, saliva, and fecal material. ### Claim 14 (depends on 13) The method of claim 13, wherein said fecal material is obtained by sampling toilet bowl water. ### Claim 15 (depends on 11) The method of claim 11, wherein said testing element is as an immunochromatographic test strip. ### Claim 16 (depends on 11) The method of claim 11, wherein said analyte of interest is an antigen or hapten, and wherein said antigen or hapten is a diagnostic indicator of a disease state in an organism.