# US 2010/0167303 A1 · Sequences diagnostic for shrimp pathogens

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- Number: US 2010/0167303 A1
- Document ID: US20100167303A1
- Status: Published application
- Kind: A1
- Application number: 12/707,762
- Filed: 2010-02-18
- Published: 2010-07-01
- Earliest claimed: 2006-10-20
- Assignees: E. I. Du Pont de Nemours and Company
- Inventors: RICHARD C. EBERSOLE; Jianzhong Zhang; Mario W. Chen; Christian Peter Lenges
- Web page: https://patentgrep.com/patent/US20100167303A1

## CPC

- C12Q 1/689: for bacteria
- C12Q: MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS (immunoassay G01N33/53); COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES

## Abstract

Primers have been isolated that are diagnostic for the detection of Vibrio harveyi. The primers are based on a portion of the Vibrio harveyi LuxR gene and may be used in primer directed amplification or nucleic acid hybridization assay methods.

## Claims

### Claim 1

A method for detecting the presence of Vibrio harveyi in a sample comprising:
(i) providing DNA from a sample suspected of containing Vibrio harveyi; and
(ii) probing the DNA with a probe derived from the isolated Vibrio harveyi diagnostic primer sequence as set forth in SEQ ID NO:1 or SEQ ID NO:2 under suitable hybridization conditions;
wherein the identification of a hybridizable nucleic acid fragment confirms the presence of Vibrio harveyi.

### Claim 2 (depends on 1)

The method for detecting the presence of Vibrio harveyi in a sample according to claim 1 wherein the probe derived from the isolated Vibrio harveyi diagnostic primer sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, and the complete complementary sequences thereof.

### Claim 3 (depends on 1)

The method according to claim 1 wherein the probe contains a replication inhibiting moiety at the 3′ end.

### Claim 4 (depends on 3)

The method according to claim 3 wherein the replication inhibiting moiety is selected from the group consisting of dideoxynucleotides, 3′ deoxynucleotides, a sequence of mismatched nucleosides or nucleotides, 3′ phosphate groups and chemical agents.

### Claim 5 (depends on 4)

The method according to claim 4 where in the 3′ deoxynucleotide is cordycepin.

### Claim 6 (depends on 1)

The method according to claim 1 wherein the method further comprises the steps of:
a) treating an environment from which the sample was obtained with a chemical treatment to kill or control the Vibrio harveyi; and
b) repeating the steps of the method of claim 1 on another sample taken from the environment in order to assess Vibrio harveyi inactivation by the chemical treatment.

### Claim 7 (depends on 1)

The method according to claim 1 wherein the method further comprises the step of: treating an environment from which the sample was obtained with a chemical treatment to kill or control the Vibrio harveyi in order to improve health and grow-out of shrimp.