# US 2010/0166709 A1 · Novel Bacteriophage and Antibacterial Composition Comprising the Same

> Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt

- Number: US 2010/0166709 A1
- Document ID: US20100166709A1
- Status: Published application
- Kind: A1
- Application number: 12/645,414
- Filed: 2009-12-22
- Published: 2010-07-01
- Earliest claimed: 2008-12-24
- Assignees: Cj Cheiljedang Corporation
- Inventors: In Hye Kang; Min Tae Park; Young Wook Cho; Soo An Shin; Hyang Choi
- Web page: https://patentgrep.com/patent/US20100166709A1

## CPC

- C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00)
- A61P 31/04: Antibacterial agents
- A61K 35/13: Tumour cells, irrespective of tissue of origin (tumour vaccines A61K39/00)
- C12N 2795/10121: Viruses as such, e.g. new isolates, mutants or their genomic sequences
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

The present invention relates to a novel bacteriophage, more particularly, a bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum. Further, the present invention relates to a composition for the prevention or treatment of infectious diseases including salmonellosis and Salmonella food poisoning caused by Salmonella Enteritidis or Salmonella Typhimurium, Fowl Typhoid caused by Salmonella Gallinarum, and Pullorum disease caused by Salmonella Pullorum, comprising the bacteriophage as an active ingredient. Furthermore, the present invention relates to a feed additive, drinking water, a cleaner and a sanitizer, comprising the bacteriophage as an active ingredient.

## Claims

### Claim 1

A novel bacteriophage that has a specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum, that belong to the morphotype group of the family Myoviridae, and that has a total genome size of 157-159 kbp and major structural proteins with sizes of 44-46 kDa, 61-63 kDa and 79-81 kDa.

### Claim 2 (depends on 1)

The bacteriophage according to claim 1, wherein the bacteriophage is identified by accession number KCCM10977P.

### Claim 3 (depends on 1)

The bacteriophage according to claim 1, wherein the bacteriophage has the morphology depicted in FIG. 1.

### Claim 4 (depends on 1)

The bacteriophage according to claim 1, wherein the bacteriophage includes one or more nucleic acid molecules selected from the group consisting of SEQ ID NO. 1, 2, 3, and 4 within the entire genome.

### Claim 5 (depends on 1)

The bacteriophage according to claim 1, wherein each PCR product has a size of 1 kbp, upon performing PCR using one or more primer sets selected from the group consisting of SEQ ID NOs. 5 and 6, SEQ ID NOs. 7 and 8, SEQ ID NOs. 9 and 10, and SEQ ID NOs. 11 and 12.

### Claim 6 (depends on 1)

The bacteriophage according to claim 1, wherein the bacteriophage has one or more properties of the following 1)-3):
1) acid-resistance in a pH range from pH 3.5 to pH 9.0;
2) heat-resistance in a temperature range from 37° C. to 60° C.; and
3) dry-resistance at 37-60° C. for 0-120 minutes.

### Claim 7 (depends on 1, 2, 3, 4, 5, 6)

A composition for the prevention or treatment of infectious diseases caused by one or more Salmonella bacteria selected from the group consisting of Salmonella Gallinarum, Salmonella Pullorum, Salmonella Typhimurium, and Salmonella Enteritidis, comprising the bacteriophage of any one of claims 1 to 6 as an active ingredient.

### Claim 8 (depends on 7)

The composition according to claim 7, wherein the infectious disease caused by Salmonella Enteritidis or Salmonella Typhimurium is salmonellosis or Salmonella food poisoning, the infectious disease caused by Salmonella Gallinarum is Fowl Typhoid, and the infectious disease caused by Salmonella Pullorum is Pullorum disease.

### Claim 9 (depends on 7)

The composition according to claim 7, wherein the composition is used as an antibiotic.

### Claim 10 (depends on 1, 2, 3, 4, 5, 6)

An animal feed or drinking water, comprising the bacteriophage of any one of claims 1 to 6 as an active ingredient.

### Claim 11 (depends on 1, 2, 3, 4, 5, 6)

A sanitizer or cleaner, comprising the bacteriophage of any one of claims 1 to 6 as an active ingredient.

### Claim 12 (depends on 1, 2, 3, 4, 5, 6)

A method for preventing or treating infectious diseases caused by one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum, using the bacteriophage of claims 1 to 6.

### Claim 13 (depends on 7)

A method for preventing or treating infectious diseases caused by one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum, using the composition of claim 7.