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AssigneeTemasek Life Sciences Laboratory Limited
InventorsZhongchao Yin, Dongsheng Tian
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The present invention generally provides a method to generate broad-spectrum resistance to bacterial blight disease in plants. More specifically, the present invention provides a method to generate broad-spectrum resistance to Xanthomonas oryzae pv. oryzae, the causal agent of bacterial blight disease of rice, and enhanced resistance to Xanthomonas oryzae pv. oryzicola, the causal agent of bacterial leaf streak of rice. Xa27, an inducible bacterial blight R gene in rice, was induced by the cognate avrXa27 gene expressed in host. Rice plants carrying the avrXa27 transgene and wild-type Xa27 gene conferred resistance to incompatible and compatible pathogens, and enhanced resistance to X. oryzae pv. oryzicola strain L8. The Xa27-mediated enhanced resistance to X. oryzae pv. oryzicola was also observed in the interaction between IRBB27 and L8 harboring pHM1avrXa27. This was further verified by the fact that the Xa27 gene in IRBB27 was induced by the avrXa27 gene in bacteria. The method can be used to engineer broad-spectrum resistance of rice to bacterial blight and enhanced resistance to bacterial leaf streak. Slight modification of this technique can be applied to control bacterial diseases in other crops.
A transgenic plant containing within its genome a first nucleic acid operably linked to a promoter that is active in the plant, wherein the first nucleic acid encodes an avrXa27 protein having the amino acid sequence set forth in SEQ ID NO:2.
A plant containing within its genome a first nucleic acid operably linked to a first promoter that is active in the plant and a second nucleic acid operably linked to a second promoter that is active in the plant, wherein the first nucleic acid encodes an avrXa27 protein having the amino acid sequence set forth in SEQ ID NO:2 and wherein the second nucleic acid encodes an Xa27 protein having an amino acid sequence set forth in SEQ ID NO:4.
A method of inducing expression of Xa27 gene in a plant which comprises expressing a nucleic acid in the plant, wherein the nucleic acid is operably linked to a promoter that is active in the plant and wherein the nucleic acid encodes an avrXa27 protein having the amino acid sequence set forth in SEQ ID NO:2.
A method of generating an enhanced and broad spectrum resistance to bacterial blight in a plant which comprises inducing the expression of Xa27 gene in a plant by expressing a nucleic acid in the plant, wherein the nucleic acid is operably linked to a promoter that is active in the plant and wherein the nucleic acid encodes an avrXa27 protein having the amino acid sequence set forth in SEQ ID NO:2.
A method of generating an enhanced resistance to bacterial leaf streak in a plant which comprises inducing the expression of Xa27 gene in a plant by expressing a nucleic acid in the plant, wherein the nucleic acid is operably linked to a promoter that is active in the plant and wherein the nucleic acid encodes an avrXa27 protein having the amino acid sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.