Yield Increase in Plants Overexpressing the SHSRP Genes
AssigneeBASF Plant Science GmbH
InventorsRodrigo Sarria-Millan, Eric R. Garr, Jamie Haertel, Damian Allen, Bryan McKersie
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Abstract
A transgenic crop plant transformed by a Serine Hydroxymethyltransferase-Like Stress-Related Polypeptide (SHSRP) coding nucleic acid, wherein expression of the nucleic acid sequence in the crop plant results in the plant's increased root growth, and/or increased yield, and/or increased tolerance to environmental stress as compared to a wild type variety of the plant Also provided are agricultural products, including seeds, produced by the transgenic crop plants. Also provided are isolated novel SHSRPs, and isolated novel nucleic acids encoding SHSRPs, and vectors and transgenic plant containing the same.
Claims48
A transgenic crop plant transformed with an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;
- c) a polynucleotide having at least 70% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;
- d) a polynucleotide encoding a polypeptide having at least 70% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;
- and
- e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.
A method of producing a transgenic crop plant containing an isolated nucleic acid encoding a polypeptide, wherein the method comprises the steps of, transforming a plant cell with an expression vector comprising the nucleic acid, and generating from the plant cell the transgenic plant that expresses the polypeptide, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as set forth in any of SEQ ED NOS as provided in Column No. 3 of Table 1 and Table 2;
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ D NOS as provided in Column No. 4 of Table 1 and Table 2;
- c) a polynucleotide having at least 70% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;
- d) a polynucleotide encoding a polypeptide having at least 70% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;
- and
- e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.
An isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;
- c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;
- d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;
- e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above;
- and
- f) a polynucleotide complementary to any of the polynucleotides of a) through d) above.
A transgenic plant transformed with an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;
- c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 2;
- d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 2;
- and
- e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above.
A recombinant expression vector comprising an isolated nucleic acid, wherein the nucleic acid comprises a polynucleotide selected from the group consisting of:
- a) a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;
- b) a polynucleotide encoding a polypeptide having a sequence as set forth in any of SEQ D NOS as provided in Column No. 4 of Table 1 and Table 2;
- c) a polynucleotide having at least 90% sequence identity to a polynucleotide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 3 of Table 1 and Table 2;
- d) a polynucleotide encoding a polypeptide having at least 92% sequence identity to a polypeptide having a sequence as set forth in any of SEQ ID NOS as provided in Column No. 4 of Table 1 and Table 2;
- e) a polynucleotide that hybridizes under stringent conditions to the complement of any of the polynucleotides of a) through d) above;
- and
- f) a polynucleotide complementary to any of the polynucleotides of a) through d) above.
Dependencies are inferred from the claim text.