US 2010/0162433 A1Published application

Plants with improved nitrogen utilization and stress tolerance

OwnerJames McLaren, Nicholas Duck +4inventors

InventorsJames McLaren, Nicholas Duck, Brian Vande Berg, Alissa Schawalder, Vadim Beilinson, Jill Hinson

Life of this patent

Estimating the term…

Loading patent…

Abstract

The present invention relates to transgenic plants that have increased nitrogen use efficiency, stress tolerance, or both and that have been transformed using a novel vector construct including nucleic acid sequences that modulate nitrogen use in plants. In various embodiments, the vector construct includes one or more nucleic acid sequences selected from the group consisting of SEQ ID NO: 2, 4, 7, 9, 11, 13, 15, 17, 22, 24, 26, 28, 30, 32, 34, 36, or 38. The invention also relates to isolated vectors for transforming plants and to antibodies used for detecting transformed plants. The invention also relates to methods of expressing in plants the nucleic acid molecules corresponding to the nucleic acid sequences that modulate nitrogen use in plants or are modulated by nitrogen conditions.

Claims21

Dependent claims

An expression vector comprising a nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence selected from the list consisting of SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, and SEQ ID NO:
38;
b) a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, or SEQ ID NO:
38, wherein said nucleotide sequence modulates nitrogen use in a plant;
c) a nucleotide sequence encoding an amino acid sequence selected from the list consisting of SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37, and SEQ ID NO:
36;
and,
(d) a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37, or SEQ ID NO:
39, wherein said nucleotide sequence modulates nitrogen use in a plant.

70 more elements

A plant, comprising a plant transformed with at least a first nucleotide sequence selected from the group consisting of:

a) a nucleotide sequence selected from the list consisting of SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, and SEQ ID NO:
38;
b) a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, or SEQ ID NO:
38, wherein said nucleotide sequence modulates nitrogen use in a plant;
c) a nucleotide sequence encoding an amino acid sequence selected from the list consisting of SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37, and SEQ ID NO:
36;
and,
(d) a nucleotide sequence encoding an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37, or SEQ ID NO:
39, wherein said nucleotide sequence modulates nitrogen use in a plant.

70 more elements

A vector construct, comprising:

a) at least a first nucleotide sequence encoding an amino acid sequence selected from the group consisting of:
i) an amino acid sequence selected from the list consisting of SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37 and SEQ ID NO:
39;
ii) an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
3, SEQ ID NO:
5, SEQ ID NO:
8, SEQ ID NO:
10, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
23, SEQ ID NO:
25, SEQ ID NO:
27, SEQ ID NO:
29, SEQ ID NO:
31, SEQ ID NO:
33, SEQ ID NO:
35, SEQ ID NO:
37 or SEQ ID NO:
39, wherein said amino acid sequence modulates nitrogen use in a plant;
iii) an amino acid sequence encoded by a nucleotide sequence selected from the list consisting of SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, and SEQ ID NO:
38;
and,
iv) an amino acid sequence encoded by a nucleotide sequence having at least 95% sequence identity to SEQ ID NO:
2, SEQ ID NO:
4, SEQ ID NO:
7, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
22, SEQ ID NO:
24, SEQ ID NO:
26, SEQ ID NO:
28, SEQ ID NO:
30, SEQ ID NO:
32, SEQ ID NO:
34, SEQ ID NO:
36, or SEQ ID NO:
38, wherein said nucleotide sequence modulates nitrogen use in a plant;
b) a 5′ DNA promoter sequence;
and,
c) a 3′ terminator sequence,
wherein the nucleotide sequence, the DNA promoter sequence, and the terminator sequence are operatively coupled to permit transcription of the nucleotide sequence.

75 more elements

Dependencies are inferred from the claim text.

Facts

Application: 11/977,768Filed 26 Oct 2007
Priority: 27 Oct 2006
Published: 24 Jun 2010
Term ends: Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 15/8271
With agronomic traits, e.g. crop yield: for stress resistance, e.g. heavy metal resistance
C12N 15/8216
For plant cells , e.g. plant artificial chromosomes: methods for controlling, regulating or enhancing expression of transgenes in plant cells
C12N 15/8261
Phenotypically and genetically modified plants via recombinant DNA technology: with agronomic traits, e.g. crop yield
Y02A 40/146
In agriculture: genetically Modified [GMO] plants, e.g. transgenic plants