Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko
AssigneeBASF Plant Science GmbH
InventorsPiotr Puzio, Oliver Bläsing, Oliver Thimm
Life of this patent
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Abstract
The invention relates generally to transformed plant cells and plants comprising an in-activated or down-regulated gene resulting in increased tolerance and/or resistance to environmental stress and increased biomass production as compared to non-transformed wild type cells and methods of producing such plant cells or plants.
Claims41
A method for producing a transgenic plant with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant, which comprises:
- a) reducing, repressing or deleting one or more activities selected from the group consisting of:
- 1-phosphatidylinositol 4-kinase, amino acid permease (AAP1), At3g55990-protein, At5g40590-protein, ATP-dependent peptidase/ATPase/nucleoside-triphosphatase/serine-type endopeptidase, DC1 domain-containing protein/protein-binding protein/zinc ion binding protein, DNA binding protein/transcription factor, hydro-lyase/aconitate hydratase, metalloexopeptidase (MAP1C), methyltransferase, nitrate transporter (ATNRT2.3), nitrate/chlorate transporter (NRT1.1), pectate lyase protein/powdery mildew susceptibility protein (PMR6), peptidase/ubiquitin-protein ligase/zinc ion binding protein (JR700), proton-dependent oligopeptide transport protein, transcription factor, and ubiquitin conjugating enzyme/ubiquitin-like activating enzyme, in a plant cell, a plant or a part thereof, and
- b) generating a transformed plant with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant and growing under conditions which permit the development of the plant.
A method for producing a transgenic plant with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant, which comprises:
- a) reducing, repressing, or deleting the activity of
- (i) a polypeptide comprising a polypeptide, a consensus sequence or at least one polypeptide motif as depicted in column 5 or 7 of Table II or of Table IV, respectively;
- or
- (ii) an expression product of a nucleic acid molecule comprising a polynucleotide as depicted in column 5 or 7 of Table I,
- (iii) or a functional equivalent of (i) or (ii);
- in a plant cell, a plant or a part thereof, and
- b) generating a transformed plant with increased tolerance and/or resistance to environmental stress and increased biomass production as compared to a corresponding non-transformed wild type plant and growing under conditions which permit the development of the plant.
An isolated nucleic acid molecule which comprises a nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule which encodes a polypeptide comprising the polypeptide shown in column 5 or 7 of Table IIB;
- b) a nucleic acid molecule which comprising a polynucleotide shown in column 5 or 7 of Table IB;
- c) a nucleic acid molecule comprising a nucleic acid sequence, which, as a result of the degeneracy of the genetic code, can be derived from a polypeptide sequence depicted in column 5 or 7 of Table IIB and having the activity represented by the protein depicted in column 5 of Table II;
- d) a nucleic acid molecule encoding a polypeptide having at least 50% identity with the amino acid sequence of a polypeptide encoded by the nucleic acid molecule of (a) or (c) and having the activity represented by the protein depicted in column 5 of Table II;
- e) a nucleic acid molecule encoding a polypeptide, which is isolated with the aid of monoclonal antibodies against a polypeptide encoded by one of the nucleic acid molecules of (a) to (c) and having the activity represented by the protein depicted in column 5 of Table II;
- f) a nucleic acid molecule encoding a polypeptide comprising the consensus sequence or a polypeptide motif shown in column 7 of Table IV and having the biological activity represented by the protein depicted in column 5 of Table II;
- g) a nucleic acid molecule encoding a polypeptide having the activity represented by a protein as depicted in column 5 of Table II;
- h) a nucleic acid molecule which comprises a polynucleotide, which is obtained by amplifying a cDNA library or a genomic library using the primers in column 7 of Table III which do not start at their 5′-end with the nucleotides ATA;
- and
- i) a nucleic acid molecule which is obtainable by screening a suitable library under stringent hybridization conditions with a probe comprising one of the sequences of the nucleic acid molecule of (a) to (c) or with a fragment of at least 17 nt of the nucleic acid molecule characterized in any one of (a) to (h) and encoding a polypeptide having the activity represented by the protein depicted in column 5 of Table II;
- or which comprises a sequence which is complementary thereto;
- whereby the nucleic acid molecule according to (a) to (i) is at least in one or more nucleotides different from the sequence depicted in column 5 or 7 of Table IA and which encodes a protein which differs at least in one or more amino acids from the protein sequences depicted in column 5 or 7 of Table IIA.
A TILLING primer for the identification of a knock out of a gene comprising a nucleic acid sequence of a nucleic acid molecule as depicted in any one column 5 or 7 of Table I.
A dominant-negative mutant of a polypeptide comprising a polypeptide as shown in column 5 or 7 of Table II.
A transgenic plant cell, plant or a part thereof wherein the activity of a protein comprising a polypeptide, a consensus sequence or a polypeptide motif as depicted in column 5 or 7 of Table II or IV or a nucleic acid molecule comprising a nucleic acid molecule as depicted in column 5 or 7 of Table I is reduced.
(canceled)
Dependencies are inferred from the claim text.