Process for purifying a polymer mixture
AssigneeSynthon BV
InventorJordy Luten
Life of this patent
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Abstract
A process of purifying copolymer peptides such as COP-1 polypeptides by ultrafiltration can be improved by treating the polypeptide solution with an acid before, or during the early stages of, the ultrafiltration. By adding the acid and/or forming an acid addition salts of the polypeptide before ultrafiltration is conducted permits for faster ultrafiltration. Likewise, adding the acid in the ultrafiltration process but before polypeptide precipitation or clogging occurs can also improve the efficiency/convenience of the ultrafiltration step.
Claims20
A process of ultrafiltration, which comprises:
- combining a reaction mixture having a pH of 10 or higher and containing a variety of copolymer polypeptides which collectively have more basic amino acids than acidic amino acids, with an aqueous acid solution to form a modified reaction mixture;
- and
- subjecting said modified reaction mixture to ultrafiltration to form a purified reaction mixture.
A process of making purified COP-1, which comprises:
- (i) adding an aqueous acid solution to a reaction mixture containing unprotected COP-1 polypeptides to obtain COP-1 acid addition salts in a modified reaction mixture;
- and
- (ii) ultrafiltering said modified reaction mixture containing said COP-1 acid addition salts to form purified COP-1 acid addition salts;
- wherein step (i) is performed before subjecting said reaction mixture containing the unprotected COP-1 polypeptides to any ultrafiltration.
In a process for purifying COP-1 polypeptides which comprises subjecting unprotected COP-1 polypeptides in a reaction mixture to ultrafiltration wherein the COP-1 polypeptides become purified in the retentate solution and the pH of the retentate decreases during the ultrafiltration, the improvement for which comprises adding an acid to the reaction mixture or retentate before or during, or both, the ultrafiltration process but not later than the retentate achieves a pH of 10.
Dependencies are inferred from the claim text.