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US 2010/0160585 A1Published application
Method and apparatus for isolating and purifying nucleic acid using a single surface
AssigneeSamsung Electronics Co., Ltd.
InventorsChang eun YOO, Sung-young JEONG, Young-rok KIM
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Abstract
Provided is a method of isolating nucleic acid from cells using a single surface, wherein a compound represented by Formula 1 is bound to the surface. Also provided are an apparatus for isolation of nucleic acids, and a bead for isolating nucleic acids.
Claims4
A bead for isolating nucleic acid from cells, comprising
- a material represented by Formula 1 below bound to the surface of the bead:
- where Z1 is a carboxyl group or an amino group;
- R1, R2, R3, R5, R6, R7, R8, R9 and R11 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxyl group, a substituted or unsubstituted C1-C20 alkyl group, a substituted or unsubstituted C1-C20 alkoxy group, a substituted or unsubstituted C2-C20 alkenyl group, a substituted or unsubstituted C6-C30 aryl group, and a substituted or unsubstituted C6-C30 aryloxy group;
- R4 is a substituted or unsubstituted C4-C20 alkyl group, a substituted or unsubstituted C4-C20 alkoxy group, a substituted or unsubstituted C4-C20 alkenyl group, a substituted or unsubstituted C6-C30 aryl group, or a substituted or unsubstituted C6-C30 aryloxy group;
- R10 is a nitrogen-containing heteroaryl or heterocyclic group of 3-30 carbon atoms;
- j, k, l and m are each independently an integer in the range of 1-10;
- and
- n is an integer in the range of 1-30,000.
5 more elements
A method of making a bead surface for isolating nucleic acid from cells, comprising:
- reacting a bead with a compound to introduce a hydrophobic functional group, wherein the hydrophobic functional group is a substituted or unsubstituted C4-C20 alkyl group, a substituted or unsubstituted C4-C20 alkoxy group, a substituted or unsubstituted C4-C20 alkenyl group, a substituted or unsubstituted C6-C30 aryl group, or a substituted or unsubstituted C6-C30 aryloxy group;
- reacting the bead with a second compound to introduce a charge switching moiety, wherein the charge switching moiety is a nitrogen-containing heteroaryl or heterocyclic group of 3-30 carbon atoms.
Dependencies are inferred from the claim text.