Methods, systems, and computer readable media for synthetic wavelength-based phase unwrapping in optical coherence tomography and spectral domain phase microscopy

A method for phase unwrapping of an optical coherence tomography (OCT) signal, the method comprising:

• acquiring raw OCT signal data;
• interpolating the raw OCT signal data to be evenly spaced in k;
• correcting for dispersion in the interpolated OCT signal;
• performing a Fourier transform on the interpolated OCT signal data;
• passing the transformed signal data through a low-pass filter to isolate the DC components of the transformed signal;
• performing an inverse Fourier transform on the isolated DC components to obtain a DC spectrum;
• dividing the raw OCT signal data by the DC spectrum to generate an interference signal;
• applying Gaussian windows to the interference signal to generate a first signal and a second signal;
• processing the first signal and the second signal;
• extracting phase information from each of the first signal and the second signal;
• and
• comparing the phase information of the first signal to the phase information of the second signal to produce unwrapped phase data, and adding 2π if the difference between the phase information of the first signal and the second signal is less than zero.

A system for phase unwrapping of an optical coherence tomography (OCT) signal comprising:

• a light source;
• a sample arm having optical elements configured to focus light from the source onto a sample;
• at least one spectrometer combined with at least one detector for acquiring OCT signal data from the sample;
• a data processor for processing the acquired OCT signal data, generating an interference signal, applying Gaussian windows to the interference signal to generate a first signal and a second signal, extracting phase information from each of the first signal and the second signal, comparing the phase information of the first signal to the phase information of the second signal to produce unwrapped phase data, and adding 2π if the difference between the phase information of the first signal and the second signal is less than zero.

A computer readable medium comprising computer executable instructions embodied in a tangible computer readable medium and when executed by a processor of a computer performs steps comprising:

• acquiring raw OCT signal data;
• interpolating the raw OCT signal data to be evenly spaced in k;
• processing the interpolated OCT signal data to obtain a DC spectrum;
• comparing the raw OCT signal data to the DC spectrum to generate an interference signal;
• applying Gaussian windows to the interference signal to generate a first signal and a second signal;
• processing the first signal and the second signal;
• extracting phase information from each of the first signal and the second signal;
• and
• comparing the phase information of the first signal to the phase information of the second signal to produce unwrapped phase data, and adding 2π if the difference between the phase information of the first signal and the second signal is less than zero;
• wherein the above steps are performed on a computer.