Methods for detecting catecholamines by mass spectrometry
AssigneeQuest Diagnostics Investments Incorporated
InventorsMichael P. Caulfield, Gloria Kwangja Lee
Life of this patent
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Abstract
Provided are methods for determining the amount of one or more of one or more of epinephrine (E), norepinephrine (NE), and dopamine (D) in a sample using mass spectrometry. The methods generally involve ionizing one or more of E, NE, and D in a sample and detecting and quantifying the amount of the ion to determine the amount of one or more of E, NE, and D in the sample.
Claims27
A method for determining in a sample the amount of one or more analytes selected from the group consisting of epinephrine, norepinephrine, and dopamine, the method comprising:
- a. subjecting the sample to ionization under conditions suitable to produce one or more ions detectable by mass spectrometry;
- wherein:
- i. if one of the one or more analytes is epinephrine, said ionization comprises generating ions with a mass to charge ratio of 166.1±0.50;
- ii. if one of the one or more analytes is norepinephrine, said ionization comprises generating ions with a mass to charge ratio of 151.9±0.50;
- and
- iii. if one of said one or more analytes is dopamine, said ionization comprises generating ions with a mass to charge ratio of 136.9±0.50;
- b. determining the amount of the one or more ions by tandem mass spectrometry;
- and
- c. using the amount of the one or more ions to determine the amount of said one or more analytes in the sample.
A method for determining the amount of one or more analytes selected from the group consisting of epinephrine, norepinephrine, and dopamine in a sample, said method comprising:
- a. subjecting the sample following purification by immobilized boronic acid extraction to ionization under conditions suitable to generate one or more ions detectable by mass spectrometry from the one or more analytes;
- b. determining the amount of one or more ions by tandem mass spectrometry;
- and
- c. using the amount of the one or more ions to determine the amount of said one or more analytes in the sample.
A method for determining the amount of one or more analytes selected from the group consisting of epinephrine and dopamine in a urine sample, said method comprising:
- a. subjecting the sample to ionization under conditions suitable to produce one or more ions detectable by mass spectrometry;
- wherein:
- i. if one of said one or more analytes is epinephrine, said ionization comprises generating ions with a mass to charge ratio of 166.1±0.50;
- and
- ii. if one of said one or more analytes is dopamine, said ionization comprises generating ions with a mass to charge ratio of 136.9±0.50
- b. determining the amount of one or more ions by mass spectrometry;
- and
- c. using the amount of the one or more ions to determine the amount of said one or more analytes in the sample.
A method for determining the amount of epinephrine, norepinephrine, and dopamine in a urine sample from the same sample injection, the method comprising:
- a. subjecting the sample to ionization under conditions suitable to produce one or more ions detectable by mass spectrometry from each analyte;
- wherein ionization comprises generating ions from the group consisting of ions with a mass to charge ratio of 166.1±0.50, 151.9±0.50, and 136.9±0.50;
- b. determining the amount of one or more ions from each analyte by mass spectrometry;
- and
- c. using the amount of the one or more ions to determine the amount of epinephrine, norepinephrine, and dopamine in the sample.
Dependencies are inferred from the claim text.