Purkinje Cell-Tropic Viral Vector
AssigneeJapan Science and Technology Agency
InventorsHirokazu Hirai, Takashi Torashima
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Abstract
The present invention relates to a Purkinje cell-tropic viral vector in which a modified L7 promoter and a therapeutic gene are operably linked to a virus-based plasmid vector.
Claims24
A cerebellar Purkinje cell-tropic vector comprising a modified L7 promoter and a foreign gene operably linked to a virus-based plasmid vector, wherein the modified L7 promoter comprises a DNA of the following a) or b):
- a) a DNA having the nucleotide sequence shown in SEQ ID NO:
- 3 or 4;
- or
- b) a DNA which hybridizes under stringent conditions to a DNA having a sequence complementary to the nucleotide sequence shown in SEQ ID NO:
- 3 or 4 and has a Purkinje cell-specific promoter activity equivalent to that of the DNA a).
A cerebellar Purkinje cell-tropic vector comprising a foreign gene operably linked to a lentiviral plasmid vector, which produces a viral protein in a state with substantially no degradation.
A method for preparing a Purkinje cell-tropic viral vector, comprising:
- operably linking a modified L7 promoter having the nucleotide sequence shown in SEQ ID NO:
- 3 or 4 and a foreign gene to a virus-based vector plasmid or operably linking a foreign gene to a lentiviral plasmid vector;
- introducing the vector plasmid into a host cell;
- and culturing the cell in a culture medium comprising a protease inhibitor to produce a virus.
A culture medium for preparing a Purkinje cell-tropic viral vector comprising a substance having a cathepsin K inhibitory activity.
A kit for preparing a Purkinje cell-tropic viral vector comprising, as component(s), a substance having a cathepsin K inhibitory activity and a cell culture medium, or a cell culture medium comprising a substance having a cathepsin K inhibitory activity.
Dependencies are inferred from the claim text.