Recombinant constructs and transgenic fluorescent ornamental fish therefrom
OwnerAlan Blake, Richard Crockett +3inventors
InventorsAlan Blake, Richard Crockett, Jeffrey Essner, Perry Hackett, Aidas Nasevicius
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Abstract
The present invention relates to the method and use of reef coral fluorescent proteins in making transgenic red, green and yellow fluorescent zebrafish. Preferably, such fluorescent zebrafish are fertile and used to establish a population of transgenic zebrafish and to provide to the ornamental fish industry for the purpose of marketing. Thus, new varieties of ornamental fish of different fluorescence colors from a novel source are developed.
Claims18
-44. (canceled)
A method of producing a transgenic fluorescent, ornamental fish, comprising the steps of:
- (a) obtaining one or more cloning vectors, each comprising one or more fluorescent genes under the control of a fish promoter and one or more enhancing elements.
- (b) producing a population of transgenic fluorescent, ornamental fish wherein one or more fish of said population comprise one or more fluorescent genes in its genome and express one or more fluorescent proteins.
A method of obtaining a population of the transgenic ornament fish with strong visible fluorescence, comprising the steps of:
- (a) producing a population of transgenic fluorescent, ornamental fish;
- (b) selecting from the population at least one fish that exhibits ubiquitous fluorescent expression;
- (c) monitoring the selected fish and continuing selecting for stable expression of strong visible fluorescence;
- and
- (d) creating of a line of fish that exhibit strong visible fluorescence.
-71. (canceled)
Dependencies are inferred from the claim text.