Method of preparing decellularized soft tissue, graft and culture material
AssigneeJapan AS Represented By President of National Card
InventorsAkio Kishida, Tsuyoshi Kimura, Hisatoshi Kobayashi, Toshiya Fujisato
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Abstract
It is intended to provide a method of preparing a decellularized soft tissue whereby swelling after an ultrahigh pressure treatment can be inhibited, etc. The above-described method of preparing a decellularized soft tissue comprises the application step wherein an ultrahigh hydrostatic pressure is applied to an isolated soft tissue in a medium to thereby disrupt cells in the soft tissue, and the removal step wherein the disrupted cells are removed. As the medium, use is made of an aqueous solution containing a water-soluble polysaccharide such as dextran.
Claims9
A method of preparing a decellularized soft tissue in which soft tissue of animal origin is decellularized, the method comprising:
- an application step of disrupting cells in the soft tissue by applying an ultrahigh hydrostatic pressure to an isolated soft tissue in a medium;
- and
- a removal step of removing the disrupted cells,
- wherein the medium is an aqueous solution containing a water-soluble polysaccharide.
Dependencies are inferred from the claim text.