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US 2010/0145154 A1Published application
Rodent Cremaster Preparation Device for Intravital Microscopy
AssigneeBayer HealthCare LLC
InventorPengxuan Liu
Life of this patent
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Abstract
Provided herein is a device useful for preparing a cremaster muscle of a specimen for intravital microscopy.
Claims22
A device useful for securing a cremaster muscle of a specimen during intravital microscopy comprising:
- a) a surgery tray,
- b) immobilizers, and
- c) a temperature-controlled cremaster muscle mount, wherein the mount comprises a temperature sensor, a heating element, a viewing plate, a well formed by a gasket for physiological buffer, a plurality of stand-offs, and a plurality of hooks and sutures,
- wherein the mount is positioned onto the surgery tray to hold the cremaster muscle of the specimen and wherein the hooks are positioned inside the well and are suitable for spreading the cremaster muscle, whereby the cremaster muscle is viewable under a microscope.
1 more element
A mounting device useful for securing a cremaster muscle of a specimen during intravital microscopy comprising:
- a) a plate,
- b) a plate mount,
- c) a plurality of stand-offs,
- d) a temperature sensor,
- e) a heating element,
- f) a gasket, wherein the gasket creates a well, and
- g) a plurality of hooks and sutures.
4 more elements
A method of preparing a cremaster muscle for intravital microscopy comprising the steps of:
- a) providing a specimen,
- b) placing the specimen on a surgery tray,
- c) immobilizing the specimen using immobilizers situated in specific positions on the
- tray,
- d) isolating the specimen's cremaster muscle from the scrotum,
- e) incising and positioning the cremaster muscle onto a temperature-controlled cremaster muscle mount,
- f) inserting hooks attached to sutures positioned in a circular array into the cremaster, and
- g) spreading the cremaster muscle using the hooks and sutures to a thin layer.
5 more elements
Dependencies are inferred from the claim text.