Method for the separation of phospholipids from phospholipid-containing materials
AssigneeMartek Biosciences Corporation
InventorJesus Ruben Abril
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Abstract
Methods are disclosed for extracting and separating polar lipids, including phospholipids, from materials containing oil, polar lipid, protein, ash, and/or carbohydrate, such as egg yolks and other phospholipid-containing materials. In particular, methods for extracting phospholipids from phospholipid-containing materials through the use of an aliphatic alcohol and control of temperature are disclosed. Using these methods, phospholipids in the aqueous liquid fraction will be efficiently separated and will precipitate readily, and can be subjected to separation for improved purity.
Claims31
A method for the separation of phospholipids from a phospholipid-containing material, comprising:
- a. combining the phospholipid-containing material and a water soluble aliphatic alcohol to form a phospholipid-containing fraction;
- b. cooling the phospholipid-containing fraction to precipitate the phospholipids.
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A method for the separation of phospholipids from a phospholipid-containing material comprising:
- (a) combining propanol and a phospholipid-containing material from a source selected from the group consisting of poultry eggs, enriched poultry eggs, microorganisms of the genus Thraustochytrium, microorganisms of the genus Schizochytrium, microorganisms of the species Crypthecodinium cohnii and mixtures thereof at a temperature of from about 35 C to about 75 C;
- (b) allowing the combination to form a water/propanol fraction enriched in phospholipids at a temperature of from about 35 C to about 75 C;
- (c) cooling the phospholipid-enriched fraction to a temperature of from about 5 C to about 35 C to precipitate the phospholipids;
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- (d) separating the precipitated phospholipids.
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A method for the separation of phospholipids from a phospholipid-containing material comprising:
- (a) combining propanol and a phospholipid-containing material from a source selected from the group consisting of poultry eggs and enriched poultry eggs at a temperature of about 60 C;
- (b) separating the combination into a phospholipid-enriched fraction and a protein fraction at a temperature of from about 35 C to about 75 C;
- (c) recovering the phospholipid-enriched fraction by centrifugation in a batch process or a continuous process, at a temperature of from about 35 C to about 75 C;
- (d) precipitating phospholipid from the phospholipid-enriched fraction at a temperature of about 25 C;
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- (e) separating the precipitated phospholipid by centrifugation or filtration.
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Dependencies are inferred from the claim text.