Use of snps for the diagnosis of a pain protective haplotype in the gtp cyclohydrolase 1 gene (gch1)
AssigneeJohann Wolfgang Goethe-Kuniversitaet Frankfurt Am Main
InventorsIrmgard Tegeder, Joern Loetsch
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Abstract
The present invention relates to an in vitro method for diagnosing a genetic predisposition or susceptibility for pain in a mammal which comprises detecting of at least one particular single nucleotide polymorphism (SNP) in a sample obtained from said mammal in the genomic locus-derived nucleic acid or fragment thereof of the locus GCH1.
Claims18
1-13. (canceled)
A high throughput automated screening system for determining a genetic predisposition or susceptibility for developing acute and/or chronic pain in a mammal, the system comprising:
- a) a high throughput genotyping means, connected to
- b) a computer processor and a computer-readable physical medium with software instructions encoded thereupon for a process, executable by said processor, said instructions comprising:
- (i) instructions for receiving data regarding the genotype of at least one single nucleotide polymorphism (SNP) in a mammal at the genomic locus of the gene GCH1, wherein said at least one SNP is selected from the group consisting of the SNPs rs8007267 G>A, rs3783641 A>T, rs8007201 T>G, rs4411417 A>G, rs752688 G>A, and rs10483639 C>G;
- (ii) instructions for comparing the genotype data received in (a) with reference genotype data regarding a protective haplotype for genetic predisposition or susceptibility for developing acute and/or chronic pain, wherein said comparing identifies whether said mammal carries a protective haplotype for genetic predisposition or susceptibility for developing acute and/or chronic pain;
- and
- (iii) outputting the result of said comparison to a user interface.
A method for diagnosing a genetic predisposition or susceptibility for developing acute and/or chronic pain in a mammal, comprising detecting in a sample obtained from said mammal at least one single nucleotide polymorphism (SNP) in a nucleic acid or fragment thereof derived from the genomic locus of the gene GCH1, wherein said at least one SNP is selected from the group consisting of the SNPs rs8007267 G>A, rs3783641 A>T, rs8007201 T>G, rs4411417 A>G, rs752688 G>A, and rs10483639 C>G.
A method of treating pain in a mammal, comprising
- a) detecting in a sample obtained from said mammal at least one single nucleotide polymorphism (SNP) in a nucleic acid or fragment thereof derived from the genomic locus of the gene GCH1, wherein said at least one SNP is selected from the group consisting of the SNPs rs8007267 G>A, rs3783641 A>T, rs8007201 T>G, rs4411417 A>G, rs752688 G>A, and rs10483639 C>G;
- and
- b) providing an analgesic substance to said mammal in a dosage adjusted on the basis of the result as obtained in step (a).
A kit, comprising at least one probe and/or set of primers for detecting at least one of the SNPs of the gene GCH1, selected from the group consisting of the SNPs rs8007267 G>A, rs3783641 A>T, rs8007201 T>G, rs4411417 A>G, rs752688 G>A, and rs10483639 C>G.
Dependencies are inferred from the claim text.