Diagnosis, prognosis and identification of potential therapeutic targets of multiple myeloma based on gene expression profiling

A method of diagnosing an individual with myeloma, comprising the steps of:

• obtaining a bone marrow sample from the individual;
• and
• determining an amplification of a gene on chromosome 1q21 either alone or in combination with a deletion of a gene on chromosome 13q14 in the sample, thereby diagnosing the individual with myeloma.

A method of identifying an individual having an aggressive form of a cancer, comprising the steps of:

• obtaining a bone marrow sample from the individual;
• and
• determining an amplification of a gene on chromosome 1q21, wherein the amplification of the gene is associated with poor survival, thereby identifying an individual the aggressive form of the cancer myeloma.

A method of screening for drugs useful in treating in treating cancer, comprising:

• contacting a sample comprising CKS1B gene or CSK1B gene product with a test compound;
• and
• determining the effect of the compound on amplification, over-expression or activity of the CKS1B gene or the CKS1B gene product, wherein an inhibitory effect of the test compound indicates that the test compound is a useful drug for treating the cancer.

A method of screening for drugs useful in treating high-risk myeloma, comprising:

• contacting a sample comprising CKS1B with a drug;
• and
• determining the inhibitory effect of the drug on amplification, over-expression or activity of CKS1B protein, thereby screening for drugs useful in treating high-risk myeloma.

A method of treating an individual having high-risk myeloma, comprising:

• administering to the individual a compound that inhibits amplification, over-expression or activity of CKS1B protein.

A method of treating an individual having an abnormal copy number of a gene found in chromosome 1q21 comprising:

• administering a compound that inhibits amplication, over-expression or activity of said gene.

A method of treating an individual having an abnormal copy number of a gene found in chromosome 1p13 comprising:

• administering a compound that inhibits deletion, under-expression or activity of said gene