Chloroplast expression of membrane proteins
OwnerHenry Daniellinventor
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Abstract
Disclosed herein are chloroplast transformation vectors constructed to enable expression and hyperaccumulation of membrane proteins in chloroplasts. Another embodiment relates to plants transformed with such vectors. Another embodiment relates to seeds and other plant tissues transformed with such vectors. Another embodiment relates to a method of increasing expression of membrane proteins in chloroplasts including transforming a plant cell with vectors described herein.
Claims13
A plant composition comprising an immunologically effective amount of a membrane protein and a plant remnant, wherein the membrane protein is heterologous respective to source of plant remnant.
A stable plastid transformation and expression vector which comprises an expression cassette comprising, as operably linked components in the 5′ to the 3′ direction of transcription, a promoter operative in said plastid, a selectable marker sequence, a heterologous polynucleotide sequence coding for a membrane protein, transcription termination functional in said plastid, and flanking each side of the expression cassette, flanking DNA sequences which are homologous to a DNA sequence of the target plastid genome, whereby stable integration of the heterologous coding sequence into the plastid genome of the target plant is facilitated through homologous recombination of the flanking sequence with the homologous sequences in the target plastid genome.
A stably transformed plant which comprises a plastid stably transformed to express a heterologous polypeptide, or the progeny thereof, including seeds, wherein said heterologous polypeptide comprises at least 90 percent identity with a membrane protein.
A plastid genome transformed to contain a polynucleotide having at least 90 percent identity to a membrane protein, said plastid genome configured so as to express said polynucleotide.
Dependencies are inferred from the claim text.