C-Terminal Domain Truncation of mGluR1a By Calpain and Uses Thereof
AssigneeUniversity of Southern California
InventorsMichel Baudry, Wei Xu
Life of this patent
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Abstract
A composition comprising a peptide or a peptidomimetic thereof that inhibits the C-terminal domain truncation of mGluR1α by calpain, wherein the peptide is 10-30 amino acids in length and contains a sequence that is at least 70% homologous to VIKPLTKSYQGSGK. Also disclosed are methods of detecting the C-terminal domain truncation of mGluR1α by calpain, methods of inhibiting the C-terminal domain truncation of mGluR1α in a neuron, and methods of identifying a compound that inhibits the C-terminal domain truncation of mGluR1α.
Claims28
A composition comprising a peptide or a peptidomimetic thereof that inhibits the C-terminal domain truncation of mGluR1α by calpain, wherein the peptide is 10-30 amino acids in length and contains a sequence that is at least 70% homologous to VIKPLTKSYQGSGK.
A method of detecting the C-terminal domain truncation of mGluR1α by calpain, comprising:
- providing a sample containing mGluR1α and calpain;
- and
- determining the presence or absence of the C-terminal domain truncation of mGluR1α by the calpain.
A method of inhibiting the C-terminal domain truncation of mGluR1α in a neuron, comprising:
- detecting the presence of the C-terminal domain truncation of mGluR1α in a neuron, and
- contacting the neuron with an agent that inhibits the C-terminal domain truncation of mGluR1α.
A method of identifying a compound that inhibits the C-terminal domain truncation of mGluR1α, comprising:
- providing a system containing calpain and mGluR1α;
- contacting the system with a test compound;
- and
- detecting the C-terminal domain truncation of mGluR1α in the system, wherein the C-terminal domain truncation of mGluR1α in the system, if less than the C-terminal domain truncation of mGluR1α in a control system that is not contacted with the test compound, indicates that the test compound is a candidate for inhibiting the C-terminal domain truncation of mGluR1α.
A method of identifying a compound that inhibits the C-terminal domain truncation of mGluR1α, comprising:
- providing a neuron;
- contacting the neuron with a test compound;
- and
- detecting the C-terminal domain truncation of mGluR1α in the neuron, wherein the C-terminal domain truncation of mGluR1α in the neuron, if less than the C-terminal domain truncation of mGluR1α in a control neuron that is not contacted with the test compound, indicates that the test compound is a candidate for inhibiting the C-terminal domain truncation of mGluR1α.
Dependencies are inferred from the claim text.