Anti-TNF Alpha Fibronectin Type III Domain Based Scaffold Compositions, Methods and Uses
AssigneeCentocor Ortho Biotech Inc.
InventorsSteven Jacobs, Karyn O'Neil
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Abstract
A protein scaffold based on a consensus sequence of the tenth fibronectin type III (FN3) repeat from human fibronectin, preferably human Tenascin, that binds to human TNFα including isolated nucleic acids that encode a protein scaffold, vectors, host cells, and methods of making and using thereof have applications in diagnostic and/or therapeutic compositions, methods and devices.
Claims23
An isolated protein scaffold based on a fibronectin type III (FN3) domain comprising an amino acid sequence derived from a consensus sequence of the Tenascin FN3 domains, wherein protein scaffold is capable of binding to human TNFα.
An isolated protein scaffold based on a fibronectin type III (FN3) domain comprising an amino acid sequence derived from a consensus sequence of the Tenascin FN3 domains having the amino acid sequence of any one of the B:C or F:G loops of SEQ ID NOS:22-168, wherein the protein scaffold forms loops at or about residues 13-16, 22-28, 38-43, 51-54, 60-64, and 75-81 and is capable of binding to human TNFα.
An isolated protein scaffold based on a fibronectin type III (FN3) domain comprising:
- a backbone portion having an amino acid sequence identical to SEQ ID NO:16 at residues 1-21, 29-74, and 82-89;
- a B:C loop portion between residues 21 and 29 of SEQ ID NO:16 having at least 75% identity to any one of SEQ ID NOS:22-77;
- and
- an F:G loop portion between residues 74 and 82 of SEQ ID NO:16 having at least 75% identity to any one of SEQ ID NOS:78-168, wherein the protein scaffold is capable of binding to human TNFα.
Any invention described herein.
Dependencies are inferred from the claim text.