An Apo-Ferredoxin Preparation, A Process For Producing Same And Uses Thereof
AssigneeYissum Research Development Company of the Hebrew University of Jeruslem Ltd.
InventorRachel Nechustai
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Abstract
A process for obtaining a pure preparation of an apo- form of an [Fe2S2] ferredoxin is provided. The process comprising, applying a solution comprising a holo- form of the [Fe2S2] ferredoxin on an anion exchange column; and eluting the [Fe2S2] ferredoxin from the anion exchange column with a solution comprising a salt, thereby obtaining the pure preparation of the apo- form of the [Fe2S2] ferredoxin. Also provided are pure preparations of apo-ferredoxin as well as methods of using same.
Claims31
A pure preparation of an apo- form of a ferredoxin, said preparation exhibiting thermal stability at about 70° C.
A process for obtaining a pure preparation of an apo- form of an [Fe2S2] ferredoxin, the process comprising:
- applying a solution comprising a holo- form of said [Fe2S2] ferredoxin on an anion exchange column;
- and
- eluting said [Fe2S2] ferredoxin from said anion exchange column with a solution comprising a salt, thereby obtaining the pure preparation of the apo- form of the [Fe2S2] ferredoxin.
Dependencies are inferred from the claim text.