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AssigneeExonhit Therapeutics SA
InventorsBruno TOCQUE, Laurent Bracco, Florence Edon, Fabien Schweighoffer
Estimating the term…
The invention concerns a method for identifying and/or cloning nucleic acid regions representing qualitative differences associated with alternative splicing events and/or with insertions, deletions located in RNA transcribed genome regions, between two physiological situations, comprising either hybridization of RNA derived from the test situation with cDNA's derived from the reference situation and/or reciprocally, or double-strand hybridization of cDNA derived from the test situation with cDNA's derived from the reference situation; and identifying and/or cloning nucleic acids representing qualitative differences. The invention also concerns compositions or banks of nucleic acids representing qualitative differences between two physiological situations, obtainable by the above method, and their use as probe, for identifying genes or molecules of interest, or still for example in methods of pharmacogenomics, and profiling of molecules relative to their therapeutic and/or toxic effects. The invention further concerns the use of dysregulation of splicing RNA as markers for predicting molecule toxicity and/or efficacy, and as markers in pharmacogenomics.
1-39. (canceled)
A method of producing an array of splice oligonucleotides, comprising depositing, on a support, single-stranded oligonucleotides having sequences that are complementary to and specific for two or more distinct junction sequences, wherein a first junction sequence is present in a first gene or nucleic acid product of said first gene and a second junction sequence is present in a second gene or nucleic acid product of said second gene, wherein each of said oligonucleotides has a length comprised between 10 and 60 nucleotides and a GC content comprised between 25 and 65%, and wherein said junction sequences correspond to regions of variability due to differential splicing;
The method of claim 64, wherein said array consists essentially of oligonucleotides having sequences that are complementary to and specific for differentially spliced sequences from said first or second gene or nucleic acid product thereof.
A method of producing a device comprising a support and single-stranded oligonucleotides of between 10 and 60 nucleotides in length attached to said support, wherein said method comprises depositing said oligonucleotides on said support, wherein said oligonucleotides comprise a first sequence that is complementary to and specific for at least one exon or intron of a first gene or nucleic acid product thereof and a second sequence that is complementary to and specific for at least one intron-exon, exon-exon, or exon-intron junction region of said first gene or nucleic acid product thereof, wherein said first and second sequences correspond to regions of variability in at least one product of said first gene due to differential splicing.
Dependencies are inferred from the claim text.