Parthenote-derived stem cells and methods of making and using them
OwnerShoukhart M. Mitalipovinventor
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Abstract
Primate parthenotes, cells derived from them, and libraries of such cells are disclosed. Additionally, methods are disclosed for making primate parthenotes, the production of embryonic cells from these parthenotes, and for differentiating the partenotes into desired cell types, including multi-potent and differentiated cells. Methods are also provided for treating diseases or conditions for which the desired cell types are beneficial. Methods to identify agents of interest using these cells are also described.
Claims37
A library comprising an isolated or purified primate parthenote or an isolated or purified cell derived from the primate parthenote, wherein the parthenote or cell comprises a set of heterozygous MHC alleles and a set of heterozygous alleles for a gene that does not encode an MHC molecule.
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A method for generating a primate partenote, comprising
- isolating a primate oocyte in metaphase II;
- incubating the primate oocyte in a first medium comprising about 1 to about 10 μM ionomycin and a first concentration of serum albumin for about 1 to about 10 minutes;
- incubating the primate oocyte in a second medium comprising a second concentration of serum albumin and 6-dimethylaminopurine, wherein the ratio of the first concentration of serum album to the second concentration of serum albumin is 1:20 to 1:40, and wherein the first concentration of serum albumin is between about 0.1 mg./ml to about 10 mg/ml;
- and
- culturing the primate oocyte in to form an embryo in vitro.
The method of claim 27, wherein the third medium is TALP/HEPEPS medium.
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Dependencies are inferred from the claim text.