Vector systems
AssigneeWisconsin Alumni Research Foundation
InventorsWaclaw Szybalski, Jadwiga Wild, Zdenka Hradecna, David Frisch, Frederick R. Blattner, Katarzyna Gromek
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Abstract
The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection.
Claims45
A vector comprising
- (a) an excisable fragment comprising an insertion site;
- (b) a first and second origin of replication;
- and
- (c) a pair of transcriptional terminators flanking the excisable fragment.
A method of inducing and expressing nucleic acid in an animal cell, the method comprising:
- (a) providing a vector comprising an insertion site, a first origin of replication a second origin of replication, and a pair of transcriptional terminators;
- (b) introducing the nucleic acid into the insertion site of the vector;
- (c) transforming at least one invasive bacterium with the vector to form at least one transformed bacterium;
- and
- (d) infecting the animal cell with said at least one transformed bacterium.
A method of inducing and expressing heterologous DNA or RNA in an animal cell, the method comprising:
- (a) providing a vector comprising an insertion site, a low-copy number origin of replication, an inducible high-copy number origin of replication, and a pair of transcriptional terminators;
- (b) introducing a heterologous DNA or RNA into the insertion site of the vector;
- (c) transforming reduced genome E. coli with the vector to form at least one transformed E. coli;
- and
- (d) infecting the animal cell with said transformed E. coli.
A reduced vector-host system comprising a host strain comprising repE, parA and parB genes and a vector that is free of repE, parA and parB genes.
Dependencies are inferred from the claim text.