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US 2010/0144544 A1Published application
Fluorescence labelling
AssigneeUniversity of Leicester
InventorsGeorge William Fraser, David J.M. Ray
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AssigneeUniversity of Leicester
InventorsGeorge William Fraser, David J.M. Ray
Estimating the term…
Fluorescence Labelling This invention generally relates to techniques for fluorescence labelling, and to methods, apparatus and computer program code for processing fluorescence signal data. A method of determining respective first and second degree-of-labelling signals for different respective first and second fluorophores associated with a common entity, the method comprising: determining a first fluorescence signal from said first and second fluorophores under first conditions; determining a second fluorescence signal from said first and second fluorophores under second conditions different to said first conditions; and determining said first and second degree-of-labelling signals for said first and second fluorophores from said first and second fluorescence signals; and wherein said determining of said first and second degree-of-labelling signals is responsive to at least one coupling value (c12; c21) representing a coupling of energy between said fluorophores.
A method of determining respective first and second degree-of-labelling signals for different respective first and second fluorophores associated with a common entity, the method comprising:
Apparatus for determining respective first and second degree-of-labelling signals for different respective first and second fluorophores associated with a common entity, the apparatus comprising:
A method of labelling an entity with a fluorophore, the method comprising
An entity labelled with a plurality of different fluorophores, respective numbers of said different fluorophores being such that a fluorescence signal (S(n)) from each said fluorophore is substantially maximised.
A kit of fluorophore labelled probes, wherein respective numbers of said different fluorophores are such that a fluorescence signal (S(n)) from each said fluorophore is substantially maximised.
A method of manufacturing a kit of fluorophore labelled probes, the method comprising:
22-36. (canceled)
A kit of fluorophore labelled probes comprising a kit of fluorophores, and wherein one or both of a set of fluorophores for said kit of fluorophore labelled probes and a degree-of-labelling of said probes by said fluorophores are selected using a fluorescence brightness figure of merit function (R) for a candidate said fluorophore of the set, said function comprising a function of i) a parameter dependent on a light creation efficiency of fluorophore, and ii) a parameter depending on a degree of self-quenching of the fluorophore, said selecting further being dependent on iii) a parameter dependent on a degree of coupling between the fluorophore and another fluorophore.
Dependencies are inferred from the claim text.