US 2010/0144055 A1 · Assays For Clinical Assessments of Disease-Associated Autoantibodies

Abstract

The disclosure provides methods of detecting autoantibodies (AAs) in biological samples. The methods use capture probes that can bind to an disease-associated antigen/AA complex and a detection probe that can bind to the AAs. The presence, absence, and/or amount of the complex may be measured, wherein the presence of the complex may be diagnostic or prognostic of a disease or medical condition. The disclosure also provides methods of simultaneously detecting AAs and antigens in biological samples. The presence, absence, and/or amount of AAs and antigens may be measured, wherein the amount of antigen present and/or the amount of autoantibody present may be diagnostic or prognostic of a particular disease or medical condition.

Claims55

Dependent claims

A method for detecting one or more disease-associated autoantibodies present in a sample from a subject comprising:

(a) contacting the sample with (i) a capture probe, wherein the capture probe comprises a first binding agent capable of specifically binding a disease-associated antigen and (ii) a detection probe comprising a second binding agent capable of specifically binding autoantibodies to the disease-associated antigen;
and
(b) detecting the presence of a complex formed between the capture probe, the disease-associated antigen, autoantibodies to the disease-associated antigen, and the detection probe, wherein the presence of the complex is indicative of one or more disease-associated autoantibodies in the sample.

A method for diagnosing or monitoring a disease or medical condition associated with autoantibodies in a subject, the method comprising:

(a) measuring the level of one or more disease-associated antigens in a sample from the subject;
(b) measuring the level of one or more disease-associated autoantibodies in the sample;
and
(c) comparing the levels of the disease-associated antigens and disease-associated autoantibodies in the sample to reference levels of the disease-associated antigens and disease-associated autoantibodies, wherein the presence, absence, or stage of a disease or medical condition is indicated by a difference between the reference levels and the levels of the disease-associated antigens and disease-associated autoantibodies in the sample.

1 more element

Dependencies are inferred from the claim text.

Assays For Clinical Assessments of Disease-Associated Autoantibodies

Life of this patent

Assays For Clinical Assessments of Disease-Associated Autoantibodies

Life of this patent

Abstract

Claims55