L-Arabinose Fermenting Yeast
AssigneeMidwest Research Institute
InventorsMin Zhang, Arjun Singh, Pirkko Suominen, Eric Knoshaug, Mary Ann Franden, Eric Jarvis
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Abstract
An L-arabinose utilizing yeast strain is provided for the production of ethanol by introducing and expressing bacterial araA, araB and araD genes. L-arabinose transporters are also introduced into the yeast to enhance the uptake of arabinose. The yeast carries additional genomic mutations enabling it to consume L-arabinose, even as the only carbon source, and to produce ethanol. Methods of producing ethanol include utilizing these modified yeast strains.
Claims34
A method for producing an L-arabinose utilizing yeast strain for the production of ethanol, comprising the steps of:
- modifying the yeast strain by introducing genes for the expression of an araA peptide, an araB peptide and an araD peptide to produce a transgenic yeast strain having the capacity to consume L-arabinose, and to produce ethanol from a material comprising L-arabinose.
An ethanol producing yeast strain, comprising at least one heterologous arabinose transporter wherein the ethanol producing yeast strain is aldose reductase (AR) deficient (AR−).
An arabinose fermenting yeast strain deposited with American Type Culture Collection (ATCC) as Accession Number PTA-8255, PTA-8256, PTA-8257, or PTA-8258.
A isolated non-conventional yeast arabinose transporter comprising the capability of adapting a conventional yeast for growth on arabinose when the arabinose transporter is included in the conventional yeast.
Dependencies are inferred from the claim text.