Pluripotency determining factors and uses thereof
AssigneeThe University of Edinburgh
InventorsIan Chambers, Austin G. Smith
Life of this patent
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Abstract
Pluripotency determining factors are described which act intracellularly and maintain a pluripotent cell in a pluripotent state in the absence of gp130 activation, which maintain or confer pluripotency of a human stem cell, which maintain or confer pluripotency of a mouse ES cell, and which maintain or confer pluripotency of a stem cell from a non-permissive strain of mice. The factors and vectors encoding or activating the factors are used to maintain and derive pluripotent cells, especially of higher mammals, including humans.
Claims21
1-92. (canceled)
A method of reprogramming or increasing the potency of a mouse or human cell comprising specifically increasing expression of a gene in the cell that encodes a factor which acts intracellularly and maintains or confers pluripotency on a cell in the absence of gp130 activation, wherein the factor is a polypeptide comprising the sequence of SEQ ID NO:
- 4, thereby reprogramming or increasing the potency of the cell.
A vector comprising a nucleotide sequence encoding a factor which acts intracellularly and maintains or confers pluripotency on a cell, wherein the factor is a polypeptide comprising the sequence of SEQ ID NO:
- 4.
A vector comprising a nucleotide sequence encoding a factor which acts intracellularly and promotes self renewal of a pluripotent cell, wherein the factor is a polypeptide comprising the sequence of SEQ ID NO:
- 4.
A vector comprising a nucleotide sequence encoding a factor which acts intracellularly and inhibits differentiation of a pluripotent cell, wherein the factor is a polypeptide comprising the sequence of SEQ ID NO:
- 4.
A cell containing a vector comprising a nucleotide sequence encoding a factor which acts intracellularly and maintains or confers pluripotency in a cell, wherein the factor is a polypeptide comprising the sequence of SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.