Composition And Method For Increasing Efficiency Of Introduction Of Target Substance Into Cell
OwnerMasato Miyake, Tomohiro Yoshikawa +2inventors
InventorsMasato Miyake, Tomohiro Yoshikawa, Eiichiro Uchimura, Jun Miyake
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Abstract
The present invention provides a method capable of improving the efficiency of introducing a target substance (e.g., DNA, polypeptides, sugars, or complexes thereof), which is difficult to introduce (particularly, transfect) into a cell in any circumstances. Particularly, the present invention provides a composition for increasing the efficiency of introducing a target substance into a cell, comprising (a) an actin acting substance. The present invention also provides a device and method using such a composition.
Claims41
A composition for increasing the efficiency of introducing a target substance into a cell, comprising:
- (a) an actin acting substance.
A kit for increasing the efficiency of introducing a target substance into a cell, comprising:
- (a) a composition comprising an actin acting substance;
- and
- (b) a gene introduction reagent.
A composition for increasing the efficiency of introducing a target substance into a cell, comprising:
- A) a target substance;
- and
- B) an actin acting substance.
A device for introducing a target substance into a cell, comprising:
- A) a target substance;
- and
- B) an actin acting substance,
- wherein the composition is fixed to a solid phase support.
A method for increasing the efficiency of introducing a target substance into a cell, comprising the steps of:
- A) providing the target substance;
- B) providing an actin acting substance;
- and
- C) contacting the target substance and the actin acting substance with the cell.
A method for increasing the efficiency of introducing a target substance into a cell, comprising the steps of:
- I) fixing a composition to a solid support, wherein the composition comprising:
- A) a target substance;
- and
- B) an actin acting substance;
- and
- II) contacting the cell with the composition on the solid support.
Dependencies are inferred from the claim text.