Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling
AssigneeCaridianbct, Inc.
InventorGlen Delbert ANTWILER
Life of this patent
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Abstract
A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves.
Claims6
A method of expanding cellular matter, said method comprising
- providing at least one bioreactor, said bioreactor having a cellular growth area and a supply area, said cellular growth area being separated from said supply area by a membrane, said membrane being adapted to inhibit migration of cells from said cellular growth area to said supply area and to permit migration of certain chemical compounds from said cellular growth area to said supply area and of certain other chemical compounds from said supply area to said cellular growth area;
- conducting a fluid containing cellular matter into said cellular growth area;
- providing oxygenated fluid to said supply area to maintain conditions conducive for cell growth in said cellular growth area;
- hermetically removing a sample containing cellular matter from a fluid recirculation path in fluid communication with said cellular growth area.
Dependencies are inferred from the claim text.