Facilitating Protein Solubility by Use of Peptide Extensions
AssigneeBrookhaven Science Associates, LLC
InventorsPaul I. Freimuth, Yian-Biao Zhang, Jason Howitt
Life of this patent
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Abstract
Expression vectors for expression of a protein or polypeptide of interest as a fusion product composed of the protein or polypeptide of interest fused at one terminus to a solubility enhancing peptide extension are provided. Sequences encoding the peptide extensions are provided. The invention further comprises antibodies which bind specifically to one or more of the solubility enhancing peptide extensions.
Claims16
A bacterial expression vector for expression of a recombinant protein or polypeptide of interest as a fusion product composed of said protein or polypeptide of interest fused at one terminus to a solubility enhancing peptide extension, said expression vector comprising a nucleic acid sequence encoding said solubility enhancing peptide extension selected from the group consisting of Peptide T7C (SEQ ID NO:
- 5), Peptide T7B (SEQ ID NO:
- 6), Peptide T7B1 (SEQ ID NO:
- 7), Peptide T7B2 (SEQ ID NO:
- 8), Peptide T7B3 (SEQ ID NO:
- 9), Peptide T7B5 (SEQ ID NO:
- 11), Peptide T7B6 (SEQ ID NO:
- 12), Peptide T7B7 (SEQ ID NO:
- 13), Peptide T7B8 (SEQ ID NO:
- 14), Peptide T7B9 (SEQ ID NO:
- 15), Peptide T7B10 (SEQ ID NO:
- 16), Peptide T7B11 (SEQ ID NO:
- 17), Peptide T7B12 (SEQ ID NO:
- 18), Peptide T7B13 (SEQ ID NO:
- 19), Peptide T7A1 (SEQ ID NO:
- 21), Peptide T7A2 (SEQ ID NO:
- 22), Peptide T7A3 (SEQ ID NO:
- 23), Peptide T7A4 (SEQ ID NO:
- 24), Peptide T7A5 (SEQ ID NO:
- 25), Peptide N1 (SEQ ID NO:
- 27), Peptide N2 (SEQ ID NO:
- 28), Peptide N3 (SEQ ID NO:
- 29), Peptide N4 (SEQ ID NO:
- 30), Peptide N5 (SEQ ID NO:
- 31), Peptide N6 (SEQ ID NO:
- 32), and Peptide N7 (SEQ ID NO:
- 33).
An antibody which binds specifically to one or more polypeptides selected from the group consisting of:
- Peptide T7C (SEQ ID NO:
- 5), Peptide T7B (SEQ ID NO:
- 6), Peptide T7B1 (SEQ ID NO:
- 7), Peptide T7B2 (SEQ ID NO:
- 8), Peptide T7B3 (SEQ ID NO:
- 9), Peptide T7B5 (SEQ ID NO:
- 11), Peptide T7B6 (SEQ ID NO:
- 12), Peptide T7B7 (SEQ ID NO:
- 13), Peptide T7B8 (SEQ ID NO:
- 14), Peptide T7B9 (SEQ ID NO:
- 15), Peptide T7B10 (SEQ ID NO:
- 16), Peptide T7B11 (SEQ ID NO:
- 17), Peptide T7B12 (SEQ ID NO:
- 18), Peptide T7B13 (SEQ ID NO:
- 19);
- Peptide T7A1 (SEQ ID NO:
- 21), Peptide T7A2 (SEQ ID NO:
- 22), Peptide T7A3 (SEQ ID NO:
- 23), Peptide T7A4 (SEQ ID NO:
- 24) and Peptide T7A5 (SEQ ID NO:
- 25), Peptide N1 (SEQ ID NO:
- 27), Peptide N2 (SEQ ID NO:
- 28), Peptide N3 (SEQ ID NO:
- 29), Peptide N4 (SEQ ID NO:
- 30), Peptide N5 (SEQ ID NO:
- 31), Peptide N6 (SEQ ID NO:
- 32) and Peptide N7 (SEQ ID NO:
- 33).
An isolated nucleic acid sequence encoding peptide extension T7C, comprising SEQ ID NO:
- 43 and one or more sequence encoding restriction endonuclease sites at its 5′ and at its 3′ termini.
Dependencies are inferred from the claim text.