Apparatus for the disruption of animal cells
AssigneeGlaxo Group Limited
InventorSohail Rashid CHOHAN
Life of this patent
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Abstract
The present invention relates to the field of purifying recombinant virus propagated in animal cells. More particularly, it relates to a method for extracting virus from virus-infected cells that have been grown in culture in order to release virus and to apparatus for extracting virus from virus-infected cultured cells using the methods as described herein.
Claims21
1-20. (canceled)
A method of extracting a virus from an animal cell infected with the virus, comprising the steps of
- a) providing cells in a suspending fluid,
- b) pressurising the suspension of cells to a pressure of from 5 psi to 1000 psi, and
- c) causing the pressurised suspension to flow through an restriction having a maximum diameter of 0.5 mm at a flow rate so that the cell membrane is ruptured.
A method of extracting a virus from an animal cell infected with the virus, comprising the steps of
- a) providing cells in a suspending fluid,
- b) pressurising the suspension of cells, and
- c) causing the pressurised suspension to flow through a conduit having a maximum diameter of 0.5 mm at a flow rate so that the cell membrane is ruptured.
An apparatus for the disruption of animal cells infected by virus in order to release viral particles which comprises:
- a) a reservoir containing a suspension of the cells in a suspension liquid,
- b) means to pressurise said suspension,
- c) a conduit which is from 2 cm to 10 cm in length,
- d) communication means between said pressurisation means and said conduit to cause the pressurised suspension to flow through the conduit, and
- e) means to receive output from the outlet end of the conduit.
Dependencies are inferred from the claim text.