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US 2010/0144013 A1Published application
Over expression of foldases and chaperones improves protein production
AssigneeDanisco US Inc.
InventorsFrits Goedegebuur, Paulien Neefe-Kruithof, Jeffrey P. Pucci, Michael Ward
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Abstract
The present teachings provide methods for increasing protein secretion, e.g., chymosin in filamentous fungi by co-expressing certain chaperone(s) and/or foldase(s). The present teachings also provide filamentous fungi containing certain chaperone(s) and/or foldase(s) and a protein of interest for increased secretion.
Claims26
A method for production of a secretable polypeptide in a filamentous fungal host comprising:
- expressing a secretion enhancing protein in a filamentous fungal host containing a secretable polypeptide, wherein the secretion enhancing protein is bip1 and the secretable polypeptide is a chymosin.
A filamentous fungal host containing
- a first polynucleotide encoding a secretion enhancing protein and
- a second polynucleotide encoding a chymosin,
- wherein the secretion enhancing protein is bip1, and
- wherein the first polynucleotide is operably linked to a first promoter and
- the second polynucleotide is operably linked to a second promoter.
2 more elements
Dependencies are inferred from the claim text.