Enzymatic Asymmetric Decarboxylation of Disubstituted Malonic Acids
AssigneeBioSpring GmbH
InventorsHüseyin Aygün, Sylvia Wojczewski, Markus Kircher, Susann Rosmus
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Abstract
A process for the stereoselective decarboxylation of malonic acid derivatives with mutated decarboxylases is disclosed.
Claims12
A process for the stereoselective decarboxylation of malonic acid derivatives of the formula (I)
- the process comprising:
- using a decarboxylase to produce a compound of the formula (II)
- wherein X is selected from the group consisting of OH, SH, C2-C10 alkyl, C1-C10 alkoxy, and NH2 and R is selected from the group consisting of aryl, heteroaryl, condensed aryl, and condensed heteroaryl radical, wherein R is substituted or unsubstituted and optionally wherein a substituent is selected from the group consisting of a halogen, alkyl, alkenyl, and alkynyl radical.
A decarboxylase comprising an amino acid sequence corresponding to SEQ ID NO:1, which has at least one mutation compared with SEQ ID NO:1, the said mutation being present at the amino acid positions selected from the group consisting of 17, 19, 22, 24, 25, 32, 41, 42, 46, 47, 53, 60, 61, 63, 68, 74, 83, 84, 85, 87, 94, 103, 105, 112, 116, 119, 121, 139, 142, 155, 168, 173, 178, 199, 201, 202, 203, 204, 205, 210, 221, 222, 224, 225, 226, 227, 228, 229, 230, 235, 238, 239, 240, and combinations thereof, wherein the amino acid(s) at said position(s) in SEQ ID NO:1 are replaced by any other amino acid, except that, at position 188, the cysteine residue is not replaced, and not more than 10 amino acids are inserted into and/or deleted from the sequence according to SEQ ID NO:1.
Dependencies are inferred from the claim text.