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OwnerThomas Buelter, Peter Meinhold +9inventors
InventorsThomas Buelter, Peter Meinhold, Reid M. Renny Feldman, Eva Eckl, Andrew Hawkins, Aristos Aristidou, Catherine Asleson Dundon, Doug Lies, Sabine Bastian, Frances Arnold, Jun Urano
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The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention.
A recombinant microorganism comprising an engineered metabolic pathway for producing isobutanol under aerobic and anaerobic conditions, wherein said recombinant microorganism produces isobutanol under anaerobic conditions at a rate higher than a parental microorganism comprising a native or unmodified metabolic pathway.
A mutant ketol-acid reductoisomerase (KARI) comprising two or more mutations or modifications at positions corresponding to amino acids selected from the group consisting of:
Dependencies are inferred from the claim text.