Method for preparing butanol through butyryl-coa as an intermediate using yeast
AssigneeBiofuelchem Co.
InventorsSang Yup Lee, Eleftherios Terry Papoutsakis, Yu-Sin Jang
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Abstract
Disclosed herein are a method for producing butanol in yeast having the ability to biosynthesize butanol using butyryl-CoA as an intermediate, the method comprises producing butyryl-CoA in yeast having a CoAT (acetyl-CoA:butyryl-CoA CoA-transferase)-encoding gene introduced thereinto, through various pathways, and then converting the produced butyryl-CoA to butanol.
Claims26
A recombinant yeast having butanol-producing ability, into which a CoAT (CoA-transferase)-encoding gene capable of converting organic acid to organic acid-CoA by transferring a CoA moiety to organic acid, is introduced.
A method for producing butyryl-CoA, the method comprises culturing yeast capable of biosynthesizing butyryl-CoA from fatty acids in a fatty acid-containing medium.
A method for producing butanol, the method comprises:
- culturing yeast capable of biosynthesizing butyryl-CoA from fatty acids in a fatty acid-containing medium to produce butanol;
- and
- recovering the produced butanol from the culture broth.
Dependencies are inferred from the claim text.