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OwnerMonika Rusnak-Müller, Oliver May +7inventors
InventorsMonika Rusnak-Müller, Oliver May, Petrus Johannes Hermsen, Henricus Martinus Maria Gerardus Straatman, Wolfgang Skranc, Wilhelmus Hubertus Joseph Boesten, Dannis Heemskerk, Ben De Lange, Georgios Sarakinos
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The present invention relates to a whole cell catalytic system for the preparation of an enantiomerically enriched α-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase and hydantoin racemase are coexpressed in a recombinant micro-organism wherein the genes coding for these three enzymes are located on a single replicon. The present invention further relates to the use of such a whole cell catalytic system according in the preparation of an enantiomerically enriched L-α-amino acid from a corresponding hydantoin.
Whole cell catalytic system for preparation of enantiomerically enriched α-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase, and hydantoin racemase are coexpressed in a recombinant microorganism;
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DNA construct comprising nucleotide sequences encoding hydantoinase, L-carbamoylase, and hydantoin racemase operationally linked to a single promoter;
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16-17. (canceled)
A compound of formula [1]
A compound of formula [2]
20-23. (canceled)
Whole cell catalytic system for preparation of enantiomerically enriched α-amino acid from a corresponding hydantoin wherein hydantoinase, L-carbamoylase, and hydantoin racemase are coexpressed in a recombinant microorganism;
Dependencies are inferred from the claim text.