Back to results
US 2010/0143979 A1Published application
Polypeptides having dna polymerase activity
AssigneeTakara Bio Inc.
InventorsYoshimi SATO, Kazue Nishiwaki, Nana Shimada, Shigekazu Hokazono, Takashi Uemori, Hiroyuki Mukai, Ikunoshin Kato
Life of this patent
Estimating the term…
Abstract
A polypeptide having a high fidelity DNA polymerase activity and thus being useful as a genetic engineering reagent; a gene encoding this polypeptide; a method of producing the polypeptide; and a method of amplifying a nucleic acid by using the polypeptide.
Claims9
A polypeptide having a DNA polymerase activity, which comprises the amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:24;
- (b) the amino acid sequence of SEQ ID NO:32;
- and
- (c) the amino acid sequence thereof in which one or several amino acids are deleted, inserted, added or substituted in said amino acid sequence.
1 more element
A method for producing a polypeptide, the method comprising:
- culturing a cell capable of producing a polypeptide, and
- collecting said polypeptide from the culture,
- wherein said polypeptide has a DNA polymerase activity, and comprises the amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:24;
- (b) the amino acid sequence of SEQ ID NO:32;
- (c) the amino acid sequence thereof in which one or several amino acids are deleted, inserted, added or substituted in said amino acid sequence.
3 more elements
A method for amplifying a nucleic acid, the method comprising:
- amplifying a nucleic acid using a polypeptide,
- wherein said polypeptide has a DNA polymerase activity, and comprises the amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:24;
- (b) the amino acid sequence of SEQ ID NO:32;
- and
- (c) the amino acid sequence thereof in which one or several amino acids are deleted, inserted, added or substituted in said amino acid sequence.
3 more elements
Dependencies are inferred from the claim text.