Monoterpenoid modifying enzymes
AssigneeThe University of York
InventorsLorenzo Caputi, Eng-Kiat Lim, Dianna Joy Bowles
Life of this patent
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Abstract
Monoterpenoid esters that have been modified to include a sugar pendant group are disclosed. Plant glycosyltransferase enzymes are provided that function to add a sugar, typically glucose, to monoterpenoids in a bioreactor for the production of glycosylated monoterpenoid esters, together with nucleic acid molecules encoding such enzymes and vectors and transgenic cells including such nucleic acid molecules.
Claims28
A transgenic plant cell wherein said cell is genetically modified by transfection or transformation with a vector that includes a nucleic acid molecule selected from the group consisting of:
- i) a nucleic acid molecule comprising a nucleic acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23;
- ii) a nucleic acid molecule that hybridizes under stringent hybridization conditions to a nucleic acid molecule as represented in SEQ ID NO:
- 1-5 and 11-23 and that encodes a glycosyltransferase that glycosylates a monoterpenoid;
- iii) a nucleic acid molecule that encodes a polypeptide comprising an amino acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23;
- and
- iv) a nucleic acid molecule that consists of a nucleic acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23.
(canceled)
4-11. (canceled)
A transgenic plant wherein said plant is genetically modified by transfection with a vector that includes a nucleic acid molecule selected from the group consisting of:
- i) a nucleic acid molecule comprising a nucleic acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23;
- ii) a nucleic acid molecule that hybridizes under stringent hybridization conditions to a nucleic acid molecule as represented in SEQ ID NO:
- 1-5 and 11-23 and that encodes a glycosyltransferase that glycosylates a monoterpenoid;
- and
- iii) a nucleic acid molecule that encodes a polypeptide comprising an amino acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23.
13-14. (canceled)
An isolated perillyl alcohol ester comprising a sugar pendant group to provide glycosylated perillyl alcohol.
18-31. (canceled)
An isolated linalool ester comprising a sugar pendant group to provide glycosylated linalool ester.
(canceled)
An isolated citronellol ester comprising a sugar pendant group to provide a glycosylated citronellol ester.
(canceled)
An isolated menthol ester comprising a sugar pendant group to provide glycosylated menthol ester.
40-42. (canceled)
An isolated geraniol ester comprising a sugar pendant group to provide glycosylated geraniol ester.
45-47. (canceled)
An isolated á terpineol ester comprising a sugar pendant group to provide glycosylated terpineol ester.
50-51. (canceled)
(canceled)
A process for the glycosylation of a monoterpenoid comprising the steps of:
- ii) providing a transgenic plant or a seed transfected with a nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- a) a nucleic acid sequence as represented in SEQ ID NO:
- 1-5 and 11-23;
- b) a nucleic acid molecule that hybridizes under stringent hybridization conditions to a nucleic acid molecule as represented in SEQ ID NO:
- 1-5 and 11-23 and that encodes a glycosyltransferase that glycosylates a monoterpenoid;
- and
- c) a nucleic acid molecule that encodes a polypeptide comprising an amino acid sequence as represented in Figure SEQ ID NO:
- 1-5 and 11-23;
- ii) cultivating said plant or seed under conditions that allow the glycosylation of a monoterpenoid with a sugar;
- and
- iii) isolating and purifying said glycosylated monoterpenoid from said plant and/or said seed.
Dependencies are inferred from the claim text.