Compositions and methods for enhancing the degradation or conversion of cellulose-containing material

A method for producing a substance, comprising:

• (A) saccharifying a cellulose-containing material with an effective amount of a cellulolytic enzyme composition comprising a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain;
• wherein the CEL7 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 2;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 1, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 2;
• wherein the CEL12 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 4;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 3, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 3;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 4;
• wherein the CEL45 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 6;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 5, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
• 5, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 5;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 6;
• wherein the CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 8;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 7, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
• 7, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70%, sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 7;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 8;
• wherein the CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 10;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 9, (ii) the cDNA sequence contained in or the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 9, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 9;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 10;
• and
• wherein the presence of the polypeptide having cellulolytic enhancing activity increases the degradation of the cellulose-containing material by the cellulolytic enzyme composition compared to the absence of the polypeptide having cellulolytic enhancing activity;
• (B) fermenting the saccharified cellulose-containing material of step (a) with one or more (several) fermenting microorganisms to produce a fermentation product;
• and
• (C) recovering the fermentation product.

A cellulolytic enzyme composition, comprising an effective amount of a polypeptide having cellulolytic enhancing activity, and one or more (several) components selected from the group consisting of a CEL7 polypeptide having endoglucanase activity, a CEL12 polypeptide having endoglucanase activity, a CEL45 polypeptide having endoglucanase activity, a CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain, and a CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain;

• wherein the CEL7 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 2;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 1, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 1, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 1;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 2;
• wherein the CEL12 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 4;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 3, (ii) the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 3, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 3;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 4;
• wherein the CEL45 polypeptide having endoglucanase activity is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70%, sequence identity to the mature polypeptide of SEQ ID NO:
• 6;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 5, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
• 5, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 5;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 6;
• wherein the CEL7 polypeptide having cellobiohydrolase activity with a cellulose binding domain is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 8;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 7, (ii) the cDNA sequence contained in the mature polypeptide coding sequence of SEQ ID NO:
• 7, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 7;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 8;
• wherein the CEL7 polypeptide having cellobiohydrolase activity without a cellulose binding domain is selected from the group consisting of:
• (a) a polypeptide comprising an amino acid sequence having at least 70% sequence identity to the mature polypeptide of SEQ ID NO:
• 10;
• (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
• 9, (ii) the cDNA sequence contained in or the genomic DNA sequence comprising the mature polypeptide coding sequence of SEQ ID NO:
• 9, or (iii) a full-length complementary strand of (i) or (ii);
• (c) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 70% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
• 9;
• and (d) a variant comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the mature polypeptide of SEQ ID NO:
• 10;
• and
• wherein the presence of the polypeptide having cellulolytic enhancing activity increases the degradation of the cellulose-containing material by the cellulolytic enzyme composition compared to the absence of the polypeptide having cellulolytic enhancing activity.