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AssigneesLeiden University, University of Padua
InventorsGerard W. Canters, Gerhild Zauner, Armand W.J.W. Tepper, Luigi Bubacco, Thijs J. Aartsma
Estimating the term…
A method of detection of an analyte in which (i) a protein comprising a moiety capable of binding the analyte and a fluorescent label is contacted with a medium suspected of containing the analyte; (ii) the analyte, if present, binds to the moiety; (iii) the protein is subjected to incident radiation to excite the protein and induce intrinsic emission therefrom; whereby the intrinsic emission from the protein is converted through Fluorescence Resonance Energy Transfer (FRET) into emission from the fluorescent label and the amount of said FRET is affected by the binding of the analyte to the moiety; and, (iv) the emission from the fluorescent label is measured; whereby the level of emission from the fluorescent label is indicative of the presence of the analyte, and wherein the protein undergoes no substantial conformational charge during the method. A kit for carrying out the method of the invention and a protein are also provided.
A method of detection of an analyte in which
A kit comprising a protein comprising a fluorescent label, an analyte, a radiation source for imposing incident radiation at a suitable wavelength for exciting the protein and inducing intrinsic emission therefrom, and a radiation detector capable of detecting the fluorescence emitted by the label, wherein the protein additionally comprises a moiety capable of binding the analyte and wherein the intrinsic emission from the protein may be converted through Fluorescence Resonance Energy Transfer (FRET) into emission from the fluorescent label, the amount of said FRET is affected by the binding of the analyte to the moiety, and wherein the binding of the analyte to the moiety does not induce a substantial conformational change in the protein.
A protein comprising a moiety capable of binding an analyte and a fluorescent label, wherein the protein is excitable to induce intrinsic emission therefrom, and the intrinsic emission is convertible through Fluorescence Resonance Energy Transfer (FRET) into emission from the fluorescent label and the amount of said FRET is affected by binding of the analyte to the moiety, and the binding of the analyte to the moiety does not induce a substantial conformational change in the protein.
Dependencies are inferred from the claim text.