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US 2010/0143922 A1Published application
Methods for reducing over-representation of fragment ends
AssigneeHelicos BioSciences Corporation
InventorDoron Lipson
Life of this patent
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Abstract
Methods for preparing fragments for nucleic acids sequence analysis that demonstrates uniform coverage across the full fragment length. The methods disclosed herein are useful for candidate gene re-sequencing wherein the detailed analysis is performed on selected, amplified regions of the genome.
Claims27
A method for reducing over-representation of nucleic acid fragment ends, comprising:
- a. blocking the 3′-OH of a nucleic acid molecule;
- b. fragmenting the nucleic acid molecule to produce one or more unblocked 3′-OH;
- c. modifying the one or more unblocked 3′-OH;
- d. anchoring the modified nucleic acid fragments to a solid support;
- and
- e. determining at least a portion of the sequence of the nucleic acid molecule.
3 more elements
A method for reducing over-representation of nucleic acid fragment ends, comprising:
- a. blocking the 3′-end of a nucleic acid molecule;
- b. fragmenting the nucleic acid molecule to produce one or more unblocked 3′-OH;
- c. modifying both 5′ ends and one or more unblocked 3′-OH;
- d. anchoring the modified nucleic acid fragments to a solid support;
- and
- e. determining at least a portion of the sequence of the nucleic acid molecule.
3 more elements
Dependencies are inferred from the claim text.