Nucleic acids probes for detection of yeast and fungal species
AssigneeNational University of Ireland, Galway
InventorsThomas Gerard Barry, Terance James Smith, Majella Maher
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Abstract
The present invention use of to a portion of the RPS7 gene or its corresponding mRNA in a diagnostic assay for fungal and yeast species and sequences for use in such assays and methods.
Claims29
A diagnostic kit for a yeast or fungal species comprising an oligonucleotide probe capable of binding to at least a portion of the RPS7 gene or its corresponding mRNA.
A method of using a nucleic acid molecule to detect a yeast or fungal species by assaying at least a portion of the RPS7 gene or its corresponding mRNA.
A method of detecting a target organism in a test sample comprising the steps of:
- (ii) Mixing the test sample with at least one oligonucleotide probe capable of binding to at least a portion of the RPS7 gene or its corresponding mRNA;
- (ii) hybridizing under a high stringency conditions any nucleic acid that may be present in the test sample with the oligonucleotide to form a probe:target duplex;
- and (iii) determining whether a probe:target duplex is present.
(canceled)
Dependencies are inferred from the claim text.