Capture of mycobacteria like micro-organisms
AssigneeMicrosens Medtech Limited
InventorsStuart Mark Wilson, Christopher John Stanley
Life of this patent
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Abstract
A method for the capture from a sample of micro-organisms having a hydrophobic surface, which method includes contacting the micro-organisms with a capture reagent, which capture reagent has both a hydrophobic character whereby the capture reagent binds the micro-organisms by hydrophobic interaction therewith and a polar character, the capture reagent either being present on a surface and capturing the micro-organisms thereto, or being present in solution, the method then further including capturing the micro-organisms to a surface by binding the capture reagent to the surface by polar interaction between the surface and the capture reagent.
Claims19
A method for the capture from a sample of micro-organisms having a hydrophobic surface, which method comprises contacting the micro-organisms with a capture reagent, which capture reagent has both a hydrophobic character whereby the capture reagent binds said micro-organisms by hydrophobic interaction therewith and a polar character, said capture reagent either being present on a surface and capturing said micro-organisms thereto, or being present in solution, said method then further comprising capturing said micro-organisms to a surface by binding said capture reagent to said surface by polar interaction between said surface and said capture reagent.
A method for the capture from a fluid sample of microorganisms having a hydrophobic surface, which method comprises contacting the micro-organisms with a soluble capture reagent which comprises poly-DADMAC whereby the capture reagent binds said micro-organisms, and capturing said micro-organisms to a surface by binding said capture reagent to said surface.
A micro-organism assay kit comprising either (a) a soluble capture reagent having both a hydrophobic character whereby the capture reagent is capable of binding a micro-organism to be detected by hydrophobic interaction therewith and a polyionic character, a substrate having a surface for capturing said microorganisms to said surface by binding said capture reagent to said surface by polar interaction between said surface and said capture reagent, or (b) a capture reagent coated on and thus immobilised upon a solid surface, said capture reagent having both a hydrophobic and polyionic character whereby the capture reagent is capable of binding a micro-organism to be detected,
- and at least one of:
- phage capable of infecting said micro-organism;
- primers for carrying out an amplification of genomic nucleic acid of said micro-organism or said phage;
- a culture medium for culturing said micro-organism;
- a stain for visualising said micro-organism for microscopic inspection;
- an antibody for binding said micro-organism;
- or—a detection reagent for use in detecting a metabolite produced upon culture of said micro-organism.
Dependencies are inferred from the claim text.